A complexin fusion clamp regulates spontaneous neurotransmitter release and synaptic growth
A complexin fusion clamp regulates spontaneous neurotransmitter release and synaptic growth
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DOI：
10.1038/nn1980
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发表时间：
2007-10-01
影响因子：
25
通讯作者：
Littleton, J. Troy
中科院分区：
文献类型：
--
作者：
Huntwork, Sarah;Littleton, J. Troy
神经元信号通过动作电位触发的突触囊泡融合和自发释放发生，尽管在缺乏钙的情况下防止突触囊泡过早胞吐的融合钳机制尚不清楚。在这里，我们证明了突触的自发释放是由复合蛋白（一种SNARE复合物结合蛋白）调节的。果蝇复合素无效突变体的分析表明，自发融合和突触的深刻过度生长显着增加，表明复合素功能作为融合钳在体内，并可能通过控制自发释放的速率来调节神经元连接的结构重塑。
Neuronal signaling occurs through both action potential triggered synaptic vesicle fusion and spontaneous release, although the fusion clamp machinery that prevents premature exocytosis of synaptic vesicles in the absence of calcium is unknown. Here we demonstrate that spontaneous release at synapses is regulated by complexin, a SNARE complex-binding protein. Analysis of Drosophila melanogaster complexin null mutants showed a marked increase in spontaneous fusion and a profound overgrowth of synapses, suggesting that complexin functions as the fusion clamp in vivo and may modulate structural remodeling of neuronal connections by controlling the rate of spontaneous release.