Changes in the distribution of extracellular matrix components during neural crest development in Xiphophorus spp. embryos

Changes in the distribution of extracellular matrix components during neural crest development in Xiphophorus spp. embryos
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剑尾鱼神经嵴发育过程中细胞外基质成分分布的变化。

DOI:
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发表时间:
1994
期刊:
影响因子:
--
通讯作者:
J. Vielkind
J. Vielkind
中科院分区:
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文献类型:
--
作者:
B. Sadaghiani;B. Crawford;J. Vielkind

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应用免疫组织化学方法研究了剑尾鱼和月牙鱼两种剑尾鱼神经嵴细胞发育和迁移过程中,细胞外基质(ECM)的两种主要成分硫酸软骨素蛋白聚糖(CSs)和纤连蛋白(FN)的分布变化。详细描述了hnk -1阳性ECM成分的发育变化,并与CSs和FN进行了比较。HNK-1抗原也被用作神经嵴细胞的标记物。在细胞迁移之前,神经嵴细胞迁移通路中存在CSs、FN和hnk -1阳性ECM的弱染色。在细胞迁移过程中,染色水平急剧上升,迁移接近完成后,染色水平再次下降。CSs的染色比FN的染色更广泛,而HNK-1的染色模式比其他两种抗体更明确地局限于迁移途径。有限公司…
The changes in distribution of chondroitin sulfate proteoglycans (CSs) and fibronectin (FN), two major components of the extracellular matrix (ECM), are described during the development and migration of neural crest cells in two Xiphophorus species offish, X. helleri (swordtail) and X. maculatus (platyfish), using immunohistochemistry. A detailed description of the developmental changes in HNK-1-positive ECM components is also provided and compared with those of CSs and FN. HNK-1 antigen was also used as a marker for the neural crest cells. Weak staining for CSs, FN, and HNK-1-positive ECM was present in the neural crest cell migration pathways prior to migration of the cells. The level of staining increased dramatically during migration of these cells and decreased again after migration was nearly completed. Staining for CSs was more widespread than staining for FN, while the HNK-1 staining pattern was more clearly restricted to the migratory pathways than those seen with the other two antibodies. The co...
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