Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA
Broiler chickens (Ross strain) lack insulin-responsive glucose transporter GLUT4 and have GLUT8 cDNA
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DOI：
10.1016/s0016-6480(03)00145-x
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发表时间：
2003-08-01
影响因子：
2.7
通讯作者：
Akiba, Y
中科院分区：
文献类型：
--
作者：
Seki, Y;Sato, K;Akiba, Y
尝试在具有高血糖和胰岛素抵抗特征的鸡中鉴定胰岛素反应性葡萄糖转运蛋白 GLUT4 和 GLUT8。使用大鼠 GLUT4 cDNA 探针进行的 Northern 印迹分析和使用针对哺乳动物 GLUT4 cDNA 中保守区域设计的引物进行的 RT-PCR 未能成功鉴定各种鸡组织中的 GLUT4 同源物。此外，使用大鼠 GLUT4 cDNA 探针进行基因组 Southern 印迹分析，无法在鸡组织中检测到 GLUT4 同源物。因此，这些数据表明鸡组织中缺乏 GLUT4 同源基因。然而，囊胚中另一种胰岛素反应性葡萄糖转运蛋白 GLUT8 是在鸡睾丸中借助 RACE（cDNA 末端快速扩增）反应鉴定出来的。鸡GLUT8由1449bp组成，编码区为482个氨基酸的蛋白质。推导的氨基酸序列与人、大鼠和小鼠GLUT8的同一性分别为58.8%、56.3%和56.8%。 RT-PCR检测鸡脑、肾、肾上腺、脾、肺、睾丸、胰腺中GLUT8 mRNA的表达；在骨骼肌、肝脏、脂肪组织和心脏中几乎检测不到。在此，我们首先报告在鸡中发现了 GLUT8，而哺乳动物中主要的胰岛素反应性转运蛋白 GLUT4 在这些动物中缺乏。我们提出这样的假设：鸡中可观察到的高血糖和胰岛素抵抗与其可能缺乏 GLUT4 有关。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Identification of insulin-responsive glucose transporter proteins, GLUT4 and GLUT8, was attempted in chickens that characteristically are hyperglycemic and insulin resistant. Northern blot analysis using rat GLUT4 cDNA probe and RT-PCR using primers designed against the conserved regions in mammalian GLUT4 cDNA were not successful in identifying GLUT4 homologue(s) in various chicken tissues. Furthermore, GLUT4 homologues could not be detected in chicken tissues by genomic Southern blot analyses using a rat GLUT4 cDNA probe. These data, therefore, suggest that the GLUT4 homologous gene is deficient in chicken tissues. However, GLUT8, another insulin-responsive glucose transporter in the blastocyst, was identified with the aid of RACE (rapid amplification of cDNA ends) reactions in the chicken testis. Chicken GLUT8 was composed of 1449 bp with a coding region for a 482 amino acid protein. The deduced amino acid sequence was 58.8, 56.3, and 56.8% identical with human, rat, and mouse GLUT8, respectively. By RT-PCR, GLUT8 mRNA expressions were detected in chicken brain, kidney, adrenal, spleen, lung, testis, and pancreas; and barely detectable in skeletal muscle, liver, adipose tissue, and heart. Here we firstly report that GLUT8 was identified in chickens, while GLUT4, a major insulin-responsive transporter in mammals, is deficient in these animals. We propose the hypothesis that the hyperglycemia and insulin resistance observable in chickens is associated with their possible deficiency of GLUT4. (C) 2003 Elsevier Science (USA). All rights reserved.