Functional Annotation and Analysis of Expressed Sequence Tags from the Hepatopancreas of Mitten Crab (Eriocheir sinensis)
Functional Annotation and Analysis of Expressed Sequence Tags from the Hepatopancreas of Mitten Crab (Eriocheir sinensis)
复制标题
中华绒螯蟹肝胰腺表达序列标签的功能注释与分析
DOI：
10.1007/s10126-008-9146-1
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发表时间：
2009-06-01
影响因子：
3
通讯作者：
Wang, Qun
中科院分区：
文献类型：
--
作者：
Jiang, Hui;Cai, Yi-Mei;Wang, Qun
由于中华绒螯蟹（Eriocheir sinensis）作为一种传统食物的流行，其密集捕捞是常见的，并导致疾病发病率的增加，给螃蟹养殖业造成灾难性的损失。E.中华绒螯蟹不仅是重要的消化器官，也是不可缺少的免疫器官。我们从E. sinensisand获得了代表1,178个unigenes的3,297个高质量表达序列标签。这些unigenes的一半以上是新的基因，为这个物种，其余的同源物在公共数据库中，这是非常重要的，为未来的功能研究。我们还研究了这些基因与免疫过程的关联，以了解肝胰腺除了代谢之外的主要功能之一。尽管我们的cDNA文库的采样标量相对较低，但我们能够证明肝胰腺转录组的几个重要特性，并鉴定出许多与免疫应答密切相关的基因。这些结果为进一步研究大肠杆菌的基因组学奠定了基础。包括转录组分析、物理作图和全基因组测序。
Due to its popularity as a traditional food, intensive harvesting of the mitten crab (Eriocheir sinensis) is common and has lead to an increase in disease incidence, resulting in catastrophic losses to crab aquaculture. The hepatopancreas ofE. sinensisis not only an important digestive organ but also an indispensable immune organ. We constructed a nonnormalized cDNA library from the hepatopancreas ofE. sinensisand acquired 3,297 high-quality expressed sequence tags representing 1,178 unigenes. More than half of these unigenes were novel genes for this species; the remaining had homologs in public databases, which is of great importance for future functional research. We also investigated the association of these genes with immune processes for insight into one of the main functions of the hepatopancreas besides metabolism. Despite the relatively low sampling scalar of our cDNA library, we were able to demonstrate several important properties of the hepatopancreatic transcriptome and identified numerous genes that were closely associated with immune responses. These results might serve as the basis for an in-depth genomics study ofE. sinensis, including transcriptome analysis, physical mapping, and whole genome sequencing.