Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector
Overexpression of the laccase gene, lcc1, in Lentinula edodes using the pChG vector
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使用 pChG 载体在香菇中过度表达漆酶基因 lcc1
DOI：
10.1016/j.myc.2019.02.004
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发表时间：
2019
期刊：
影响因子：
1.4
通讯作者：
Hirano Tatsuya
中科院分区：
文献类型：
--
作者：
Sato Toshitsugu;Suzuki Yuumi;Naito Masayoshi;Minami Aika;Suzuki Naoyuki;Yaegashi Kaori;Hirano Tatsuya
香菇分泌漆酶(LCC：EC 1.10.3.2)，一种工业上有用的酶。本研究将香菇3-磷酸甘油醛脱氢酶基因启动子驱动的香菇LCC基因lcc1导入香菇，并在香菇中表达。获得的转化子的LCC活性是宿主菌株的2倍，RT-PCR证实了重组LCC1的表达。香菇是一种白腐菌，俗称香菇，是日本最重要的食用菌之一。它还可以降解木质素，木质素是木材中一种有弹性的可分解成分。已知白腐菌能分泌漆酶(EC 1.10。3.2)，一种木质素降解酶(Lundell，Makela，&Hilden，2010；Rodriguez&Toca-Herrera，2007)。漆酶是一种酚氧化酶，是一种具有广泛底物特性的工业酶(Lundell等人，2010；Mate&Alcalde，2017；Rodríguez&Toca Herrera，2006)。据报道，乳杆菌分泌型漆酶对染料的有效脱色和对酚类内分泌干扰物的分解(Nagai，Sato，&Enei，2004,2002；Sato等，2009)。在乳杆菌分泌的漆酶中，Lcc1是最具特性的，也是唯一在营养菌丝培养中分泌的漆酶(Nagai等人，2002；Sakamoto等人，2008)。然而，在液体培养中香菇的漆酶产量是
Lentinula edodes secretes laccase (Lcc: EC 1.10. 3.2), an industrially useful enzyme. In this study, we introduced and expressed the L. edodes Lcc gene, lcc1, driven by L. edodes glyceraldehyde-3-phosphate dehydrogenase gene promoter into L. edodes. The resulting transformants showed 2-fold Lcc activity than that of the host strain, and expression of the recombinant lcc1 was confirmed by RT-PCR.© 2019 The Mycological Society of Japan. Published by Elsevier BV All rights reserved.Lentinula edodes, a white rot fungus commonly known as the shiitake mushroom, is one of the most important edible mushrooms in Japan. It can also degrade lignin, a resilient decomposable constituent of wood in nature. White rot fungi are known to secrete laccase (EC 1.10. 3.2), a lignin-degrading enzyme, from the hyphae (Lundell, Makela, & Hilden, 2010; Rodríguez & Toca-Herrera, 2007). Laccase is a phenol oxidase and an industrially valuable enzyme that has a wide range of substrate specificities (Lundell et al., 2010; Mate & Alcalde, 2017; Rodríguez & Toca Herrera, 2006). Effective decoloration of dyes and decomposition of phenolic endocrine disrupters by secreted laccases from L. edodes has been reported (Nagai, Sato, & Enei, 2004, 2002; Sato et al., 2009). Among the laccases secreted by L. edodes, Lcc1 is the most wellcharacterized and is the only one secreted in vegetative mycelial cultures (Nagai et al., 2002; Sakamoto et al., 2008). However, laccase production in L. edodes in liquid culture (Nagai et al., 2002) is