Xenograft models of premalignant breast disease
Xenograft models of premalignant breast disease
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DOI：
10.1023/a:1009577811584
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发表时间：
2000-10-01
影响因子：
2.5
通讯作者：
Miller, FR
中科院分区：
文献类型：
--
作者：
Miller, FR
在许多组织中识别出具有分级严重性的发育不良和增生性增殖性病变，并且通常怀疑其为癌前病变，也就是说，具有进一步进展为原位癌和浸润性癌的高风险。然而，几乎没有成功开发出任何器官的癌前病变异种移植模型。人癌前乳腺疾病的良好模型将导致在异种移植物中类似于高风险人乳腺疾病的病变，并且随着时间的推移零星地进展为浸润性癌症。在本章中，使用乳腺组织块和上皮细胞建立异种移植物和人类乳腺上皮细胞系，形成癌前病变异种移植病变的发展进行了描述。MCF10AT细胞不仅形成在异种移植物中持续存在并零星进展为癌的简单分化的导管，而且形成类似于增殖性疾病的中间增殖性病变，而不具有增生、不典型增生和原位癌。
Dysplastic and hyperplastic proliferative lesions with graded severity of atypia are recognized in a number of tissues and are generally suspected to be premalignant, that is to say at high risk for further progressing to carcinoma in situ and invasive cancer. However, few xenograft models of premalignancy for any organ sine have been successfully developed. A good model of human premalignant breast disease would lead to lesions which resemble high risk human breast disease in xenografts and sporadically progress to invasive cancer with time. In this chapter the use of breast tissue pieces and epithelial cells for establishment of xenografts and the development of human breast epithelial cell lines that form premalignant xenograft lesions are described. MCF10AT cells not only form simple differentiated ducts which persist in xenografts and sporadically progress to carcinoma, but also form intermediate proliferative lesions resembling proliferative disease without atypia, atypical hyperplasia, and carcinoma in situ.