Microbiological Contamination atWorkplaces in a Combined Heat and Power (CHP) Station Processing Plant Biomass
Microbiological Contamination atWorkplaces in a Combined Heat and Power (CHP) Station Processing Plant Biomass
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DOI：
10.3390/ijerph14010099
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发表时间：
2017-01-01
影响因子：
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通讯作者：
Gutarowska, Beata
中科院分区：
文献类型：
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作者：
Szulc, Justyna;Otlewska, Anna;Gutarowska, Beata
该研究的目的是评估植物生物质加工热电站 (CHP) 的微生物污染情况。我们在空气中发现了 2.42 x 10(3) CFU/m(3) 的细菌和 1.37 x 10(4) CFU/m(3) 的真菌；生物量中细菌为 2.30 x 10(7) CFU/g，真菌为 4.46 x 10(5) CFU/g；过滤式面罩呼吸器 (FFR) 中的细菌为 1.61 x 10(2) CFU/cm(2)，真菌为 2.39 x 10(1) CFU/cm(2)。采用培养方法，在空气中发现了8属的嗜温细菌和7属的真菌；生物量中细菌和真菌各10属； FFR 分别为 2 和 5。宏基因组分析 (Illumina MiSeq) 显示 FFR 上存在 46 个细菌属和 5 个真菌属，包括潜在病原体热带念珠菌、大肠杆菌、普雷沃氏菌属、曲霉属、青霉属。使用扫描电子显微镜 (SEM) 证实了微生物在 FFR 上形成生物膜的能力。我们还鉴定了生物量和 FFR 中的次生代谢物，包括烟曲霉碱、奎西青宁、杂色菌素和 3-硝基丙酸，它们可能对人类有毒。由于存在潜在病原体和霉菌毒素，应监测热电联产工作场所的微生物污染水平。
The aim of the study was to evaluate the microbial contamination at a plant biomass processing thermal power station (CHP). We found 2.42 x 10(3) CFU/m(3) of bacteria and 1.37 x 10(4) CFU/m(3) of fungi in the air; 2.30 x 10(7) CFU/g of bacteria and 4.46 x 10(5) CFU/g of fungi in the biomass; and 1.61 x 10(2) CFU/cm(2) bacteria and 2.39 x 10(1) CFU/cm(2) fungi in filtering facepiece respirators (FFRs). Using culture methods, we found 8 genera of mesophilic bacteria and 7 of fungi in the air; 10 genera each of bacteria and fungi in the biomass; and 2 and 5, respectively, on the FFRs. Metagenomic analysis (Illumina MiSeq) revealed the presence of 46 bacterial and 5 fungal genera on the FFRs, including potential pathogens Candida tropicalis, Escherichia coli, Prevotella sp., Aspergillus sp., Penicillium sp.). The ability of microorganisms to create a biofilm on the FFRs was confirmed using scanning electron microscopy (SEM). We also identified secondary metabolites in the biomass and FFRs, including fumigaclavines, quinocitrinines, sterigmatocistin, and 3-nitropropionic acid, which may be toxic to humans. Due to the presence of potential pathogens and mycotoxins, the level of microbiological contamination at workplaces in CHPs should be monitored.