Kribbella monticola sp. nov., a novel actinomycete isolated from soil.
Kribbella monticola sp. nov., a novel actinomycete isolated from soil.
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DOI：
10.1099/ijsem.0.003007
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发表时间：
2018-11
影响因子：
2.8
通讯作者：
Wei-bo Song;Liping Duan;Junwei Zhao;Shanwen Jiang;Xiaowei Guo;W. Xiang;Xiangjing Wang
中科院分区：
文献类型：
--
作者：
Wei-bo Song;Liping Duan;Junwei Zhao;Shanwen Jiang;Xiaowei Guo;W. Xiang;Xiangjing Wang
从黑龙江省香炉山土壤中分离到一株放线菌，命名为NEAU-SW 521 T。16 S rRNA基因分析结果表明，NEAU-SW 521 T为Kribbella属的一个成员。16 S rRNA基因系统发育分析和gyrB、rpoB、relA、recA和atpD基因的多位点序列分析结果均表明该菌株与白色克里贝拉DSM 15500 T形成了一个进化枝（99.16%），人参异源克里贝拉JCM 16928 T（98.96%），Kribbella catacumanti JCM 14968T（98.82%）、圣家克里贝拉JCM 14969 T（98.62%）、七台河克里贝拉NEAU-GQTH 2 - 3 T（98.61%）和朝鲜克里贝拉JCM 10977 T（98.47%）。细胞壁含有作为主要二氨基酸的II-二氨基庚二酸，全细胞水解产物为核糖、葡萄糖和半乳糖。主要极性脂质为二磷脂酰甘油、磷脂酰胆碱、磷脂酰甘油、磷脂酰肌醇和一种未鉴定的磷脂。主要的甲基萘醌是MK-9（H4）。主要脂肪酸为异C16：0和反异C15：0。这些化学分类学数据支持NEAU-SW 521 T隶属于Kribbella属。DNA G+C含量为67.8mol%。此外，通过串联的基因遗传距离，结合DNA-DNA杂交结果和一些表型特征，可以清楚地区分菌株。因此，建议NEAU-SW 521 T代表Kribbella属的一个新种，并建议将其命名为Kribbellamonticola sp. nov.。模式菌株为NEAU-SW 521 T（=CGMCC 4.7465T=DSM 105770 T）。
A novel actinobacterium, designated strain NEAU-SW521T, was isolated from soil collected from Xianglu Mountain, Heilongjiang province, north PR China. The results of analysis of the 16S rRNA gene indicated that NEAU-SW521T represented a member of the genus Kribbella. The results of phylogenetic analyses using the 16S rRNA gene and multilocus sequence analysis using the concatenated gene sequences of the gyrB, rpoB, relA, recA and atpD genes all indicated that the strain formed a clade with Kribbella alba DSM 15500T (99.16 %), Kribbella ginsengisoli JCM 16928T (98.96 %), Kribbella catacumbae JCM 14968T (98.82 %), Kribbella sancticallisti JCM 14969T (98.62 %), Kribbella qitaiheensis NEAU-GQTH2-3T (98.61 %) and Kribbella koreensis JCM 10977T (98.47 %). The cell wall contained ll-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose, glucose and galactose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid. The predominant menaquinone was MK-9(H4). Major fatty acids were iso-C16 : 0 and anteiso-C15 : 0. These chemotaxonomic data supported the affiliation of NEAU-SW521T to the genus Kribbella. The DNA G+C content was 67.8 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA-DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that NEAU-SW521T represents a novel species of the genus Kribbella, for which the name Kribbellamonticola sp. nov. is proposed. The type strain is NEAU-SW521T (=CGMCC 4.7465T=DSM 105770T).