Capsaicin sensitive neurons role in the inflamed TMJ acute nociceptive response of female and male rats
Capsaicin sensitive neurons role in the inflamed TMJ acute nociceptive response of female and male rats
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DOI：
10.1016/j.physbeh.2007.01.002
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发表时间：
2007-04-23
影响因子：
2.9
通讯作者：
Kramer, Phillip R.
中科院分区：
文献类型：
--
作者：
Bellinger, Larry L.;Spears, Robert;Kramer, Phillip R.
计算机化的进餐模式分析，更具体地说，进餐持续时间，最近已被用作颞下颌关节（TMJ）伤害性感受的非侵入性生物标记物。在发炎的TMJ中负责伤害性反应的细胞可以包括辣椒素（CAP）敏感神经元。为了测试CAP敏感神经元在急性伤害性反应中的作用，首先用溶剂或CAP（一种已知可破坏大部分C纤维的药物）对雄性和雌性大鼠进行了腹腔给药。第二，56天后，将大鼠分为四组：新生大鼠注射赋形剂，并用和不用完全弗氏佐剂（CFA）处理。治疗组包括新生儿非CAP溶剂治疗且未注射TMJ（CON）;溶剂治疗且注射TMJ CIA（CFA）; CAP治疗且未注射（CAP）;以及CAP治疗且注射CIA（CAP+CFA）。在注射后两天分析进餐模式。在非CAP给药大鼠中，CFA注射延长了雄性大鼠给药后第1天和第2天的进餐时间，但雌性大鼠仅在第1天。CAP治疗雄性和雌性大鼠防止显着延长CIA诱导的进餐时间。CAP治疗减弱CFA诱导的降钙素基因相关肽在三叉神经节中表达的增加，在男性和女性中相似。数据表明CAP敏感神经元部分负责与CIA给药相关的急性伤害性反应的传递，并表明性别可以影响发炎TMJ区域的伤害性感受。(c)2007爱思唯尔公司All rights reserved.
Computerized meal pattern analysis, and more specifically meal duration, has recently been used as a non-invasive biological marker of nociception in the temporomandibular joint (TMJ). Cells responsible for the nociceptive response in the inflamed TMJ may include capsaicin (CAP) sensitive neurons. To test the role of CAP sensitive neurons in acute nociceptive responses first, male and female rats were treated neonatally with vehicle or CAP, an agent known to destroy a majority of C fibers. Second, after 56 days the rats were divided into four groups: neonatal vehicle-injected and treated with and without complete Freund's adjuvant (CFA). Treatment groups included neonatal non-CAP vehicle treated and TMJ not-injected (CON); vehicle treated and TMJ CIA injected (CFA); CAP-treated and not-injected (CAP); and CAP-treated and CIA injected (CAP+CFA). Meal patterns were analyzed for two days after injection. CFA-injection in non-CAP-treated rats lengthened meal duration on the first and second day after treatment in the males, but only on the first day in the females. CAP treatment in male and female rats prevented significant lengthening of meal duration induced by CIA. CAP treatment attenuated the CFA-induced increase in calcitonin gene-related peptide expression in the trigeminal ganglia similarly in males and females. The data suggests CAP-sensitive neurons are responsible, in part, for transmission of acute nociceptive responses associated with CIA administration and suggest gender can affect nociception in the inflamed TMJ region. (c) 2007 Elsevier Inc. All rights reserved.