Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels.
Stem Cells in Aggregate Form to Enhance Chondrogenesis in Hydrogels.
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DOI：
10.1371/journal.pone.0141479
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Detamore MS
中科院分区：
文献类型：
--
作者：
Sridharan B;Lin SM;Hwu AT;Laflin AD;Detamore MS
有各种令人兴奋的水凝胶技术正在探索软骨再生医学。我们的总体目标是探索在这些应用中使用聚集形式的干细胞是否可能是有利的。3D干细胞聚集体具有很大的前景，因为它们可以重现体内骨骼组织凝聚，这是在2D培养中通常观察不到的特性。我们考虑了两种不同的干细胞来源，人脐带沃顿氏胶质细胞（hWJC，目前正在临床试验中使用）和大鼠骨髓间充质干细胞（rBMSC）。本研究的目的是比较细胞表型、聚集体大小和聚集体数量对通用水凝胶（琼脂糖）平台中软骨形成分化的影响。尽管是不同的细胞来源，但rBMSC和hWJC聚集体在生物合成和软骨形成方面一致地优于细胞悬浮液对照组。较高的细胞密度有利地影响生物合成，并且聚集体的数量对软骨形成有积极影响。因此，我们建议使用水凝胶的研究人员考虑使用聚集形式的细胞来增强软骨形成性能。
There are a variety of exciting hydrogel technologies being explored for cartilage regenerative medicine. Our overall goal is to explore whether using stem cells in an aggregate form may be advantageous in these applications. 3D stem cell aggregates hold great promise as they may recapitulate the in vivo skeletal tissue condensation, a property that is not typically observed in 2D culture. We considered two different stem cell sources, human umbilical cord Wharton’s jelly cells (hWJCs, currently being used in clinical trials) and rat bone marrow-derived mesenchymal stem cells (rBMSCs). The objective of the current study was to compare the influence of cell phenotype, aggregate size, and aggregate number on chondrogenic differentiation in a generic hydrogel (agarose) platform. Despite being differing cell sources, both rBMSC and hWJC aggregates were consistent in outperforming cell suspension control groups in biosynthesis and chondrogenesis. Higher cell density impacted biosynthesis favorably, and the number of aggregates positively influenced chondrogenesis. Therefore, we recommend that investigators employing hydrogels consider using cells in an aggregate form for enhanced chondrogenic performance.