Expression of VEGFR-3 and 5′-nase in regenerating lymphatic vessels of the cutaneous wound healing
Expression of VEGFR-3 and 5′-nase in regenerating lymphatic vessels of the cutaneous wound healing
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DOI：
10.1002/jemt.20082
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发表时间：
2004-06-15
影响因子：
2.5
通讯作者：
Kato, S
中科院分区：
文献类型：
--
作者：
Ji, RC;Miura, M;Kato, S
血管内皮生长因子-C（VEGF-C）是一种特异性淋巴管生成因子，为淋巴管发育和再生的研究提出了新的问题和前景。对小鼠伤口愈合皮肤进行5 '-Nase和VEGFR-3（VEGF-C的受体）组织化学染色，以区分毛细血管和毛细血管，并分析淋巴管生成。在伤后3-5天的伤口中，皮下组织5 '-Nase阳性淋巴管中不均匀地出现抗VEGFR-3免疫阳性信号。真皮及皮下组织散在分布有少量小圆形及不规则的血管样结构，表达VEGFR-3。在伤口的第7天和第15天之间，许多积累的血管被5 '-Nase和PECAM-1染色，沿着伤口边缘不规则地沿着延伸。血管性血友病因子表达于皮下组织血管内皮细胞和毛细血管内皮细胞。淋巴管的超微结构变化发生在不同的阶段，从淋巴管样结构到新形成的淋巴管，具有极薄和锯齿状的壁。淋巴管的内皮细胞最终以典型的细胞间连接为特征，其沉积有VEGFR-3和5 '-Nase-cerium的反应产物，但缺乏VEGF-C表达。本研究结果表明，VEGF-C诱导的淋巴管生成沿着伤口愈合边缘从皮下到真皮沿着发生，特别是在真皮-皮下过渡区，有利于再生淋巴管的生长。(C)2004 Wiley-Liss，Inc.
The vascular endothelial growth factor-C (VEGF-C), a specific lymphangiogenic growth factor, raises new questions and perspectives in studying lymphatic development and regeneration. Wound healing skins in mice were processed for 5'-nucleotidase (5'-Nase) and VEGFR-3 (the receptor of VEGF-C) histochemical staining to distinguish lymphatics from blood capillaries and to analyze lymphangiogenesis. In the wounds of 3-5 days after injury, anti-VEGFR-3 immunopositive signals unevenly appeared in 5'-Nase-positive lymphatic vessels in the subcutaneous tissue. A few small circular and irregular lymphatic-like structures with VEGFR-3 expression scattered in the dermal and subcutaneous tissues. Between days 7 and 15 of the wounds, numerous accumulated vasculatures were stained for 5'-Nase and PECAM-1, extending irregularly along the wound edge. Von Willebrand factor was expressed in the endothelial cells of blood vessels and lymphatics in the subcutaneous tissue. Ultrastructural changes of lymphatic vessels developed at different stages, from lymphatic-like structures to newly formed lymphatic vessels with an extremely thin and indented wall. Endothelial cells of the lymphatic vessel were eventually featured by typical intercellular junctions, which deposited with reaction products of VEGFR-3 and 5'-Nase-cerium but lacked VEGF-C expression. The present findings indicate that VEGF-C-induced lymphangiogenesis occurs from the subcutaneous to the dermis along the wound healing edge, especially in the dermal-subcutaneous transitional area, favorable to growth of regenerating lymphatic vessels. (C) 2004 Wiley-Liss, Inc.