Protective effect of tanshinone IIA on rat kidneys during hypothermic preservation
Protective effect of tanshinone IIA on rat kidneys during hypothermic preservation
复制标题
丹参酮IIA对大鼠低温保存肾脏的保护作用
DOI：
10.3892/mmr.2011.639
复制
发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Ling, Shucai
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoming;He, Daqiang;Ling, Shucai
肾脏保存是一个普遍的问题，因为缺血/再灌注（I/R）损伤在肾移植过程中仍然是一个未解决的问题。丹参酮 IIA 是中药丹参的有效成分之一，据报道具有多种生化活性，包括预防 I/R 损伤。因此，确定介导丹参酮HA保护肾脏的特定分子途径对于相关患者具有重要价值。在本研究中，将大鼠分为两组，分离肾脏并分别保存在两种溶液中，一组含有 Celsior 溶液，另一组含有添加到 Celsior 溶液中的丹参酮 HA。保存0、24、48 h后测定超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量，免疫组化染色检测CHOP和caspase-12表达，并进行实时定量逆转录聚合酶链反应分析。丹参酮IIA保存肾脏24、48 h后SOD活性显着升高，MDA含量降低（P
Renal preservation is a universal problem since ischemia/reperfusion (I/R) injury remains an unresolved issue during the procedure of renal transplantation. Tanshinone IIA, one of the effective components of the traditional Chinese medicine Danshen, was reported to exhibit a variety of biochemical activities, including protection against I/R injury. Therefore, identifying the specific molecular pathway mediating tanshinone HA protection of renal preservation would be of great value to the patients concerned. In this study, rats were divided into two groups and the kidneys were isolated and preserved in two solutions separately, one with Celsior solution and the other with tanshinone HA additionally added to the Celsior solution. The superoxide dismutase (SOD) activity and the quantity of malonaldehyde (MDA) were measured, the expression of CHOP and caspase-12 were assessed by immunohistochemistry staining, and real-time quantitative reverse transcription-polymerase chain reaction analysis was performed after 0, 24 and 48 h of preservation. A significant increase in the activities of SOD and a decrease in the quantity of MDA were observed in the kidneys preserved with tanshinone IIA at 24 and 48 h (P