Decolourization and detoxification of textile industry wastewater by the laccase-mediator system
Decolourization and detoxification of textile industry wastewater by the laccase-mediator system
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DOI：
10.1016/j.jhazmat.2009.10.079
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发表时间：
2010-03-15
影响因子：
13.6
通讯作者：
Mechichi, Tahar
中科院分区：
文献类型：
--
作者：
Khlifi, Rim;Belbahri, Lassad;Mechichi, Tahar
研究了在不添加漆酶介质和有漆酶介质的情况下，用特罗吉曲霉漆酶对纺织工业废水脱色脱毒的效果。即使在测试的最高酶浓度下，仅漆酶也不能有效地对废水进行脱色：在9u /mL的反应混合物中，脱色率不到10%。为了增强废水脱色，在0 ~ 1mm的浓度范围内测试了几种潜在的漆酶介质。大多数潜在介质增强了废水的脱色，以1-羟基苯并三唑（HBT）最有效。考察了pH、温度、染料浓度等影响酶活性的理化参数。在pH值为5、温度为50℃、1mm HBT存在的条件下，20%的废水脱色效果最佳。采用Microtox法对粗废水、漆酶- hbt处理废水和漆酶-乙酰丁香酮处理废水的毒性进行了评价。只有漆酶-乙酰丁香酮处理的出水无毒；原油和漆酶- hbt处理的废水保留毒性。(C) 2009 Elsevier B.V.版权所有
Decolourization and detoxification of a textile industry effluent by laccase from Trametes trogii in the presence and the absence of laccase mediators was investigated. Laccase alone was not able to decolourize the effluent efficiently even at the highest enzyme concentration tested: less than 10% decolourization was obtained with 9 U/mL reaction mixture. To enhance effluent decolourization, several potential laccase mediators were tested at concentrations ranging from 0 to 1 mM. Most potential mediators enhanced decolourization of the effluent, with 1-hydroxybenzotriazol (HBT) being the most effective. The effect of several physico-chemical parameters that could influence enzyme activity, such as pH, temperature and dye concentration was tested. Optimal decolourization occurred with 20% effluent at pH 5, a temperature of 50 degrees C, and in the presence of 1 mM HBT.The toxicities of crude, laccase-HBT treated and laccase-acetosyringone treated effluent were evaluated using the Microtox assay. Only laccase-acetosyringone treated effluent was not toxic; crude and laccase-HBT treated effluent retained toxicity. (C) 2009 Elsevier B.V. All rights reserved.