RAT-LIVER OMEGA-AMIDASE - KINETIC EVIDENCE FOR AN ACYL-ENZYME INTERMEDIATE
RAT-LIVER OMEGA-AMIDASE - KINETIC EVIDENCE FOR AN ACYL-ENZYME INTERMEDIATE
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DOI：
10.1021/bi00762a007
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
HERSH, LB
中科院分区：
文献类型：
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作者：
HERSH, LB
材料与方法对位取代的戊二酸苯酯通过Gaetjens和Morawitz（1960）的方法制备。如前所述制备α-酮戊二酸乙酯和戊二酰胺酸酯（Hersh，1971 a）。使用前，将盐酸羟胺和盐酸甲胺从乙醇-乙醚中重结晶。所有其他试剂均通过化学方法获得，并且在使用前通常进行重结晶或再蒸馏。f得克萨斯大学生物化学系，
Materials and MethodsReagents. Para-substituted phenyl glutarates were prepared by the method of Gaetjens and Morawitz (1960). Ethyl a-ketoglutarate and glutaramate were prepared as previously de-scribed (Hersh, 1971a). Hydroxylamine hydrochloride and methylamine hydrochloride were recrystallized from ethanol-ether prior to use. All other reagents were obtained commer-cially and were generally recrystallized or redistilled before use. f From the Department of Biochemistry, The University of Texas,