Temperature sensitivities of metazoan and pre-metazoan Src kinases.
Temperature sensitivities of metazoan and pre-metazoan Src kinases.
复制标题
后生动物和前后生动物 Src 激酶的温度敏感性。
DOI：
10.1016/j.bbrep.2020.100775
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发表时间：
2020
影响因子：
2.7
通讯作者：
Miller,WTodd
中科院分区：
文献类型：
--
作者：
Miller,WTodd
来自不同物种的同源酶显示出与生物体所遇到的生理和环境温度相关的功能特征。在这项研究中，我们研究了非受体酪氨酸激酶Src的温度敏感性。我们比较了c-Src和来自单细胞真核生物的两种Src激酶的温度依赖性，即choanoflagellateMonosiga brevicollisand filastereanCapsaspora owczarzaki。Metazoan C - src表现出温度敏感性，在30℃和37℃时具有高活性。这种敏感性是由底物结合和最大速度的变化驱动的，它依赖于底物中酪氨酸周围的氨基酸序列。当用c-Src显示温度依赖性磷酸化的肽进行测试时，单细胞Src激酶的酶促率在测试温度上的变化要小得多。数据表明，单细胞Src激酶相对于后生动物c-Src具有温度补偿功能，这与对环境的进化适应一致。
Homologous enzymes from different species display functional characteristics that correlate with the physiological and environmental temperatures encountered by the organisms. In this study, we have investigated the temperature sensitivity of the nonreceptor tyrosine kinase Src. We compared the temperature dependencies of c-Src and two Src kinases from single-celled eukaryotes, the choanoflagellateMonosiga brevicollisand the filastereanCapsaspora owczarzaki. Metazoan c-Src exhibits temperature sensitivity, with high activity at 30 °C and 37 °C. This sensitivity is driven by changes in substrate binding as well as maximal velocity, and it is dependent on the amino acid sequence surrounding tyrosine in the substrate. When tested with a peptide that displays temperature-dependent phosphorylation by c-Src, the enzymatic rates for the unicellular Src kinases show much less variation over the temperatures tested. The data demonstrate that unicellular Src kinases are temperature compensated relative to metazoan c-Src, consistent with an evolutionary adaptation to their environments.