Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori
Determination by LC-MS of Juvenile Hormone Titers in Hemolymph of the Silkworm, Bombyx mori
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DOI：
10.1271/bbb.120883
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发表时间：
2013-05-01
影响因子：
1.6
通讯作者：
Shiotsuki, Takahiro
中科院分区：
文献类型：
--
作者：
Furuta, Kenjiro;Ichikawa, Akio;Shiotsuki, Takahiro
采用液相色谱-质谱联用技术（LC-MS）测定家蚕血淋巴中保幼激素（JH）1、11和1,11的含量。用甲醇和三氟乙酸处理JH以转化成JH甲氧基醇（JH-MH）。JH-MH分析条件的关键是向洗脱液中加入5 μ M乙酸钠。观察到每个JH-MH作为具有良好灵敏度的钠加合离子。改进后的方法使家蚕血淋巴中JH Ⅰ、Ⅱ和Ⅲ的滴定从3龄到蛹早期都能得到监测。JH Ⅰ在3、4龄幼虫蜕皮后立即出现高峰。JH I滴度在第1日龄急剧下降，蜕皮前降至最低，但在第5日龄开始时没有出现峰值，直至化蛹后才有明显上升。
Juvenile hormone (JH) 1,11 and Ill in the hemolymph of the silkworm, Bombyx mori were quantified by liquid chromatography-mass spectrometry (LC-MS). JHs were treated with methanol and trifluoroacetic acid to convert into JH methoxyhydrines (JH-MHs). The key to the analytical condition for JH-MHs was the addition of 5 mu M sodium acetate to the eluting solution. Each JH-MH was observed as the sodium adduct ion with good sensitivity. This improved method enabled the titration of JH I, II and III in hemolymph of the silkworm to be monitored from the 3rd instar through to the early pupal stage. A peak of JH I was observed immediately after ecdysis in the 3rd and 4th instar stages. The JH I titer sharply decreased on day 1 and reached the lowest level before ecdysis, but there was no peak at the beginning of the 5th stadium, and no apparent increase was observed until pupation.