HprK(Xcc) is a serine kinase that regulates virulence in the Gram-negative phytopathogen Xanthomonas campestris
HprK(Xcc) is a serine kinase that regulates virulence in the Gram-negative phytopathogen Xanthomonas campestris
复制标题
HprK(Xcc) 是一种丝氨酸激酶，可调节革兰氏阴性植物病原体野油菜黄单胞菌的毒力
DOI：
10.1111/1462-2920.14740
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发表时间：
2019
影响因子：
5.1
通讯作者：
Lu Guang-Tao（陆光涛）
中科院分区：
文献类型：
--
作者：
Li Rui-Fang（李瑞芳);Cui Ping（崔萍）;Wei Ping-Zhen（韦萍真）;Liu Xing-Yan（刘兴艳）;Tang Ji-Liang（唐纪良）;Lu Guang-Tao（陆光涛）
The HprK serine kinase is a component of the phosphoenolpyruvate phosphotransferase system (PTS) of bacteria that generally regulates catabolite repression through phosphorylation/dephosphorylation of the PTS protein PtsH at a conserved serine residue. However, many bacteria do not encode a complete PTS or even have an HprK homologue.Xanthomonas campestrispv.campestris(Xcc) is a pathogen that cause black rot disease in crucifer plants and one of the few Gram‐negative bacteria that encodes a homologue of HprK protein (herein HprKXcc). To gain insight into the role of HprKXccand other PTS‐related components inXccwe individually mutated and phenotypically assessed the resulting strains. Deletion ofhprKXccdemonstrated its requirement for virulence and other diverse cellular processes associated including extracellular enzyme activity, extracellular‐polysaccharide production and cell motility. Global transcriptome analyses revealed the HprKXcchad a broad regulatory role inXcc. Additionally, through overexpression, double gene deletion and transcriptome analysis we demonstrated thathprKXccshares an epistatic relationship withptsH. Furthermore, we demonstrate that HprKXccis a functional serine kinase, which has the ability to phosphorylate PtsH. Taken together, the data illustrates the previously unappreciated global regulatory role of HprKXccand previously uncharacterized PTS components that control virulence in this pathogen.
