T lymphocyte line specific for thyroglobulin produces or vaccinates against autoimmune thyroiditis in mice.
T lymphocyte line specific for thyroglobulin produces or vaccinates against autoimmune thyroiditis in mice.
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甲状腺球蛋白特异性 T 淋巴细胞系在小鼠体内产生或接种疫苗以预防自身免疫性甲状腺炎。
DOI：
10.4049/jimmunol.131.5.2316
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发表时间：
1983
影响因子：
4.4
通讯作者：
I. Cohen
中科院分区：
文献类型：
--
作者：
R. Maron;R. Zerubavel;A. Friedman;I. Cohen
我们研究了Ly-1+ T淋巴细胞系细胞特异性反应甲状腺球蛋白（Tg），从小鼠引发小鼠Tg佐剂。静脉内接种少至10（5）个细胞系就足以在完整、辐照或无胸腺裸小鼠中引起严重和长期的甲状腺炎。疾病是独立的循环Tg抗体，这表明抗Tg T淋巴细胞可以引起甲状腺炎的抗体的援助。用非致甲状腺原性牛Tg佐剂免疫表面健康的小鼠，可分离到致甲状腺原性T淋巴细胞，提示在外源抗原免疫过程中，自身免疫效应T淋巴细胞可能是隐性产生的。最后，一个单一的静脉注射接种抗Tg T淋巴细胞系细胞减毒辐射接种小鼠完全对随后的发展产生的自身免疫性甲状腺炎无论是主动免疫Tg或被动转移的完整的线细胞。免疫小鼠甲状腺炎的炎症性病变的保护仍然产生高滴度的Tg抗体在主动免疫。因此，疫苗接种特异性抑制甲状腺致炎性T淋巴细胞，但不是辅助T淋巴细胞所需的Tg自身抗体的生产。
We investigated Ly-1+ T lymphocyte line cells specifically reactive to thyroglobulin (Tg) that were isolated from mice primed with mouse Tg in adjuvant. Intravenous inoculation of as few as 10(5) line cells was sufficient to cause severe and prolonged thyroiditis in recipient mice that were intact, irradiated, or athymic nudes. Disease was independent of circulating Tg antibodies, suggesting that anti-Tg T lymphocytes could cause thyroiditis unaided by antibodies. Thyroiditogenic T lymphocytes could be isolated as cell lines from apparently healthy mice that had been immunized with non-thyroiditogenic bovine Tg in adjuvant, which indicates that autoimmune effector T lymphocytes may develop covertly in the course of immunization with foreign antigens. Finally, a single i.v. inoculation of anti-Tg T lymphocyte line cells attenuated by irradiation vaccinated mice completely against subsequent development of autoimmune thyroiditis produced either by active immunization to Tg or by passive transfer of intact line cells. Vaccinated mice that were protected from inflammatory lesions of thyroiditis still produced high titers of Tg antibodies in response to active immunization. Thus, vaccination specifically inhibited thyroiditogenic T lymphocytes but not helper T lymphocytes required for the production of Tg autoantibodies.