The effect of vitamin D on sperm motility and the underlying mechanism
The effect of vitamin D on sperm motility and the underlying mechanism
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维生素D对精子活力的影响及其机制
DOI：
10.4103/aja.aja_105_18
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发表时间：
2019-07-01
影响因子：
2.9
通讯作者：
Yao, Bing
中科院分区：
文献类型：
--
作者：
Jueraitetibaike, Kadiliya;Ding, Zheng;Yao, Bing
维生素D缺乏症是世界各地常见的健康问题。因此，我们评估了精液质量与血清和精浆维生素D水平的关系，并通过在体外用1，25（OH）2D孵育精子来研究其机制。222名男性被纳入我们的研究。维生素D的检测采用电化学发光法。通过密度梯度离心分离用于体外实验的精子。血清25（OH）D与精液量和精浆果糖呈正相关。精浆25（OH）D水平与血清25（OH）D水平无相关性，但与精子密度呈负相关，与精液量和精子动力学值呈正相关。在体外，精子动力学参数与1，25（OH）2D孵育后增加，特别是在0.1 nmol l−1浓度下孵育30分钟后。在这些孵育条件下，精子的向上迁移随三磷酸腺苷（ATP）浓度的增加而显著增加。环磷酸腺苷（cAMP）浓度和蛋白激酶A（PKA）活性均升高，PKA抑制剂N-[2-（p-溴肉桂酰胺）乙基]-5-异喹啉磺酰胺二盐酸盐（H89）可逆转ATP产生的增加。胞浆钙离子浓度和烟酰胺腺嘌呤二核苷酸（NADH）浓度均升高，而线粒体钙单向转运体（MCU）抑制剂钌360（Ru 360）不能逆转ATP产生的增加。因此，精浆维生素D可能参与调节精子活力，1，25（OH）2D可能通过cAMP/PKA途径和细胞内钙离子的增加促进ATP的合成来增强精子活力。
Vitamin D deficiency is a common health issue around the world. We therefore evaluated the associations of semen quality with both serum and seminal plasma vitamin D levels and studied the mechanisms underlying these by incubating spermatozoa with 1,25(OH)2D In vitro. Two hundred and twenty-two men were included in our study. Vitamin D was detected using an electrochemiluminescence method. Spermatozoa used for In vitro experiments were isolated by density gradient centrifugation. Positive relationships of serum 25(OH)D with semen volume and seminal plasma fructose were identified. Seminal plasma 25(OH)D level showed no relationship with serum 25(OH)D level, while it was inversely associated with sperm concentration and positively correlated with semen volume and sperm kinetic values. In vitro, sperm kinetic parameters increased after incubation with 1,25(OH)2D, especially upon incubation for 30 min with it at a concentration of 0.1 nmol l−1. Under these incubation conditions, the upward migration of spermatozoa increased remarkably with increasing adenosine triphosphate (ATP) concentration. The concentration of cyclic adenosine monophosphate (cAMP) and the activity of protein kinase A (PKA) were both elevated, and the PKA inhibitor, N-[2-(p-Bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) reversed the increase of ATP production. The concentrations of cytoplasmic calcium ions and nicotinamide adenine dinucleotide (NADH) were both enhanced, while mitochondrial calcium uniporter (MCU) inhibitor, Ruthenium 360 (Ru360) did not reverse the increase of ATP production. Therefore, seminal plasma vitamin D may be involved in regulating sperm motility, and 1,25(OH)2D may enhance sperm motility by promoting the synthesis of ATP both through the cAMP/PKA pathway and the increase in intracellular calcium ions.