Coping with cold:: The genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125
Coping with cold:: The genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125
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DOI：
10.1101/gr.4126905
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发表时间：
2005-10-01
期刊：
影响因子：
7
通讯作者：
Danchin, A
中科院分区：
文献类型：
--
作者：
Médigue, C;Krin, E;Danchin, A
相当一部分生命是在温度低于 15 摄氏度的海洋中发育的。人们对在这些条件下选择的适应性特征知之甚少。我们对快速生长的南极细菌 Pseudoalteromonas haloplanktis TAC125 的基因组序列进行了分析。我们发现它通过增加双氧清除同时删除产生活性氧的整个途径来应对低温下氧溶解度的增加。消耗双氧的脂质去饱和酶实现了对氧气的保护和使膜流体的脂质合成。 A haloplanktis 开发了一种避免活性氧产生的显着策略，消除了普遍存在的钼蝶呤依赖性代谢。嗜盐浮游藻蛋白质组揭示了嗜冷菌特有的一致氨基酸使用偏差，始终倾向于适应天冬酰胺，这是一种容易使蛋白质老化的残留物。除了其独创性之外，盐生浮游生物还与海洋生物不同，其第二条染色体招募了质粒复制起点。
A considerable fraction of life develops in the sea at temperatures lower than 15 degrees C. Little is known about the adaptive features selected under those conditions. We present the analysis of the genome Sequence of the fast growing Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. We find that it copes with the increased Solubility of oxygen at low temperature by multiplying dioxygen scavenging while deleting whole pathways producing reactive oxygen species. Dioxygen-consuming lipid desaturases achieve both protection against oxygen and synthesis of lipids making the membrane fluid. A remarkable strategy for avoidance of reactive oxygen species generation is developed by A haloplanktis, with elimination of the ubiquitous molybdopterin-dependent metabolism. The A haloplanktis proteome reveals a concerted amino acid usage bias specific to psychrophiles, consistently appearing apt to accommodate asparagine, a residue prone to make proteins age. Adding to its originality, A haloplanktis further differs from its marine Counterparts with recruitment of a plasmid origin of replication for its second chromosome.