Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance
Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance
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DOI：
10.1007/978-1-60327-999-4_7
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Sampath, Rangarajan
中科院分区：
文献类型：
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作者：
Ecker, David J.;Massire, Christian;Sampath, Rangarajan
我们描述了一种新的技术，用于微生物的分子基因分型，使用商业上称为Ibis T5000的平台。该技术将多位点聚合酶链反应（PCR）与电喷雾电离/质谱（PCR/ESI-MS）相结合，旨在在适用于医院或参考诊断实验室或公共卫生环境的平台上对分离的菌落或原始患者标本进行快速，高通量和精确的数字分析。PCR/ESI-MS方法可测量微生物的数字分子特征，从而实现实时流行病学监测和疫情调查。这一技术将有助于了解传染性有机体的传播途径，并能在以前无法实现的时限内采取适当的干预措施。
We describe a new technology for the molecular genotyping of microbes using a platform known commercially as the Ibis T5000. The technology couples multilocus polymerase chain reaction (PCR) to electrospray ionization/mass spectrometry (PCR/ESI-MS) and was developed to provide rapid, high-throughput, and precise digital analysis of either isolated colonies or original patient specimens on a platform suitable for use in hospital or reference diagnostic laboratories or public health settings. The PCR/ESI-MS method measures digital molecular signatures from microbes, enabling real-time epidemiological Surveillance and outbreak investigation. This technology will facilitate understanding of the pathways by which infectious organisms spread and will enable appropriate interventions on a time frame not previously achievable.