Hzf regulates adipogenesis through translational control of C/EBPα
Hzf regulates adipogenesis through translational control of C/EBPα
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DOI：
10.1038/emboj.2008.76
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发表时间：
2008-05-21
期刊：
影响因子：
11.4
通讯作者：
Sugimoto, Masataka
中科院分区：
文献类型：
--
作者：
Kawagishi, Hiroyuki;Wakoh, Takeshi;Sugimoto, Masataka
脂肪细胞分化需要一个明确的基因表达程序，其中转录因子C/EBP α （CCAAT/增强子结合蛋白）具有核心功能。在这里，我们发现Hzf（造血锌指），一个先前确定的p53转录靶点，调节C/EBP α的表达。Hzf在前脂肪细胞系分化过程中被诱导，短发夹RNA对其抑制会破坏脂肪形成。在Hzf缺失的情况下，由于C/EBP α mRNA的翻译减少，其表达严重受损。Hzf与C/EBP α mRNA的3'非翻译区相互作用以增强其翻译。综上所述，这些发现强调了Hzf在脂肪形成调控级联中的关键作用。
Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBP alpha (CCAAT/enhancer-binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBP alpha expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBP alpha is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3' untranslated region of C/EBP alpha mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.