Development of nanofiber-reinforced hydrogel scaffolds for nucleus pulposus regeneration by a combination of electrospinning and spraying technique
Development of nanofiber-reinforced hydrogel scaffolds for nucleus pulposus regeneration by a combination of electrospinning and spraying technique
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DOI：
10.1002/app.38316
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发表时间：
2013-04
影响因子：
3
通讯作者：
Anna Thorvaldsson;J. Silva-Correia;J. Oliveira;R. Reis;P. Gatenholm;P. Walkenström
中科院分区：
文献类型：
--
作者：
Anna Thorvaldsson;J. Silva-Correia;J. Oliveira;R. Reis;P. Gatenholm;P. Walkenström
本文提出了一种高效制备纳米纤维增强水凝胶支架的新方法，以提高材料的力学性能和结构完整性。该方法是基于水凝胶的气刷喷涂和纳米纤维的静电纺丝的结合。气刷喷涂的可控性增强，扩大规模的潜力增加。该方法成功地用静电纺聚己内酯纳米纤维增强了结冷胶水凝胶。光学和流变学评价表明，纳米纤维掺入量、结冷胶浓度和氯化钙（交联剂）浓度等参数可以调节材料的性能。少量纳米纤维的掺入具有增强作用，使水凝胶具有类似于人类髓核（NP）的流变特性。该方法具有灵活性，具有设计NP组织再生等支架的潜力。（
In this work a new method is presented to efficiently produce hydrogel scaffolds reinforced with nanofibers to show enhanced mechanical properties and improved structural integrity. The method is based on a combination of air brush spraying of a hydrogel and electrospinning of nanofibers. With air brush spraying the controllability is enhanced and the potential for scale-up increased. The developed method was used to successfully reinforce gellan gum hydrogels with electrospun polycaprolactone nanofibers. Optical and rheological evaluations were performed and showed that parameters such as the amount of incorporated nanofibers, gellan gum concentration and calcium chloride (crosslinker) concentrations could be used to modulate material properties. Incorporation of a small amount of nanofibers had a reinforcing effect and resulted in a hydrogel with rheological properties similar to the human nucleus pulposus (NP). The method is flexible and carries potential for designing scaffolds for e. g. NP tissue regeneration. (