Differential expression of L- and T-type calcium channels between longitudinal and circular muscles of the rat myometrium during pregnancy
Differential expression of L- and T-type calcium channels between longitudinal and circular muscles of the rat myometrium during pregnancy
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DOI：
10.1159/000082333
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发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Kitamura, K
中科院分区：
文献类型：
--
作者：
Ohkubo, T;Kawarabayashi, T;Kitamura, K
本研究的目的是评估妊娠期大鼠子宫肌层纵肌层和环肌层两类钙通道mRNA表达的差异。采用比较动力学 RT/PCR 方法检测大鼠子宫肌层纵向和环状平滑肌细胞中编码 L 型（α1C）和 T 型（α1G、α1H 和 α1I）钙通道 mRNA 表达的变化。在妊娠过程中，纵向肌中α1C mRNA表达呈N形变化，但环状肌中妊娠中期后简单增加。 α1G 和 α1H 的 mRNA 在纵向和环状平滑肌中均表达，但 α1I 的 mRNA 不表达。在纵向肌中，妊娠期间α1H mRNA的变化与α1C mRNA的变化相似，但α1G mRNA的表达仅在足月（第22天）时发生显着变化。在环状肌中，α1H mRNA 表达在怀孕期间的任何阶段都很稳定，但 α1G mRNA 在第 15 天和足月时显着增加。电压依赖性钙通道 mRNA 表达与妊娠期间环状肌增殖或肥大之间没有观察到任何关系。这些结果表明（a）在怀孕期间，L型通道的表达水平动态变化，并且可能直接有助于细胞兴奋性的调节；（b）怀孕期间增加的T亚型在纵向和环状肌细胞之间有所不同，尽管它们的功能仍不清楚。版权所有 (C) 2005 S. Karger AG，巴塞尔。
The aim of this study was to evaluate the difference in the expression of mRNA of two types of calcium channels between longitudinal and circular muscle layer of rat myometrium during pregnancy. Changes in the expressions of the mRNA encoding L-type (alpha1C) and T-type (alpha1G, alpha1H, and alpha1I) calcium channels in longitudinal and circular smooth muscle cells of the rat myometrium were examined using a comparative kinetic RT/PCR method. During the course of pregnancy, alpha1C mRNA expression showed an N-shaped change in longitudinal muscle, but simply increased after mid-pregnancy in circular muscles. The mRNAs for alpha1G and alpha1H, but not that for alpha1I, were expressed in both longitudinal and circular smooth muscle. In longitudinal muscle, the change in alpha1H mRNA was similar to that in alpha1C mRNA during gestation, but the expression of alpha1G mRNA changed significantly only at term (day 22). In circular muscle, alpha1H mRNA expression was stable at any stage during pregnancy, but alpha1G mRNA significantly increased on day 15 and at term. No relationship was observed between voltage-dependent calcium-channel mRNA expressions and either proliferation or hypertrophy of circular muscle during pregnancy. These results show ( a) that during pregnancy, the expression levels of L-type channels change dynamically, and it may contribute directly to the regulation of cell excitability, and (b) that the T-subtype that increases during pregnancy differs between longitudinal and circular muscle cells, although their functions remain unclear. Copyright (C) 2005 S. Karger AG, Basel.