Quantitative analysis of pyridinium crosslinks of collagen in the synovial fluid of patients with rheumatoid arthritis using high-performance liquid chromatography
Quantitative analysis of pyridinium crosslinks of collagen in the synovial fluid of patients with rheumatoid arthritis using high-performance liquid chromatography
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DOI：
10.1007/bf01419951
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发表时间：
1996-05-01
影响因子：
4
通讯作者：
Stein, G
中科院分区：
文献类型：
--
作者：
Muller, A;Hein, G;Stein, G
吡啶鎓交联是成熟硬组织胶原蛋白降解的重要且明确的生物标志物。采用梯度离子配对反相高效液相色谱法同时测定25例类风湿关节炎（RA）患者滑液（SF）样品中的两种交联剂。SF中吡啶啉（Pyd）和脱氧吡啶啉（Dpyd）的平均+/- SD水平分别为107.7 +/- 182.3 nmol/l和4.8 +/- 8.3 nmol/l。Pyd/Dpyd比率，其指示从软骨而不是骨释放的Pyd的量，达到30.8 +/-29.5。该值显著高于相同患者的尿液或血清。这些数据表明RA患者关节软骨破坏增加，SF中II型胶原片段释放增加。此外，SF中的交联水平反映了相当大的个体间差异，表明降解的胶原材料量存在显著的个体差异。
The pyridinium crosslinks are important and definite biomarkers of mature hard tissue collagen degradation. A gradient ion-paired reversed-phase high-performance liquid chromatographic method was used for the simultaneous determination of both crosslinks in synovial fluid (SF) samples of 25 patients with rheumatoid arthritis (RA). The mean +/- SD levels of pyridinoline (Pyd) and deoxypyridinoline (Dpyd) in SF were 107.7 +/- 182.3 nmol/l and 4.8 +/- 8.3 nmol/l, respectively. The Pyd/Dpyd ratio, which indicates the amount of Pyd released from cartilage rather than bone, amounted to 30.8 +/- 29.5. This value is significantly higher than in urine or serum of the same patients. These data suggest increased destruction of joint cartilage in patients with RA and the release of collagen II fragments in SF. In addition, the levels of the crosslinks in SF reflect considerable interindividual variation, indicating substantial individual differences in the amount of collagenous material that is degraded.