ABSORPTION AND OPTICAL ROTATORY DISPERSION OF 7 TRINUCLEOSIDE DIPHOSPHATES
ABSORPTION AND OPTICAL ROTATORY DISPERSION OF 7 TRINUCLEOSIDE DIPHOSPHATES
复制标题
DOI：
10.1016/s0022-2836(65)80080-8
复制
发表时间：
1965-01-01
影响因子：
5.6
通讯作者：
TINOCO, I
中科院分区：
文献类型：
--
作者：
CANTOR, CR;TINOCO, I
通过胰核糖核酸酶消化酵母RNA和随后的色谱分离制备ApApC、ApApU、GpGpC、GpApU、ApGpU和GpGpU。研究了这六种三核苷二磷酸和ApApA在pH 1，7和11.5时的旋光色散和减色性。此外，ORD和hypochromism已计算出相应的磷酸二核苷的性质，假设只有最近邻核苷碱基之间的相互作用是重要的。计算结果与实验结果吻合得很好，表明二核苷磷酸和三核苷二磷酸的相邻碱基具有相似的构象。结果表明，ApApA、ApApC、ApGpU、GpApU和ApApApU在pH7时均存在碱基堆积现象。GpGpC是在pH 1下显示出大量堆叠的唯一化合物，ApApC和ApApU在pH 11.5下堆叠。GpApU和ApGpU的计算和实验ORD在pH 7时非常不同。这意味着核苷酸序列可以仅通过光学测量来确定。
ApApC, ApApU, GpGpC, GpApU, ApGpU and GpGpU were prepared by pancreatic ribonuclease digestion of yeast RNAand subsequent chromatographic separations. The optical rotatory dispersion and hypochromism of these six trinucleoside diphosphates and ApApA have been studied at pH 1, 7 and 11.5. In addition, the ORD and hypochromism have been calculated from corresponding dinucleoside phosphate properties, assuming that only nearest-neighbor interaction between nucleoside bases is important. The calculations agree very well with experiment, suggesting that neighboring bases in dinucleoside phosphates and trinucleoside diphosphates have similar conformations. The results indicate that ApApA, ApApC, ApGpU, GpApU and ApApU have stacked bases at pH7. GpGpC is the only compound to show much stacking at pH 1, and ApApC and ApApU are stacked at pH 11.5. Both the calculated and experimental ORD of GpApU and ApGpU are very different at pH 7. This means that nucleotide sequence can be determined by optical measurements alone.