Selection of peptide ligands for human placental transcytosis systems using in vitro phage display.
Selection of peptide ligands for human placental transcytosis systems using in vitro phage display.
复制标题
使用体外噬菌体展示选择人胎盘转胞吞系统的肽配体。
DOI：
10.1007/978-1-61779-012-6_8
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Pauletti,GiovanniM
中科院分区：
文献类型：
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作者：
Basha,Saleem;Vaidhyanathan,Shruthi;Pauletti,GiovanniM
Fetal pharmacotherapy generally relies on nonspecific biodistribution of therapeutic agents to the unborn child following drug administration into the maternal circulation system. Physiologically, transfer of polar, high-molecular weight solutes across the placenta is facilitated by a specialized, vesicular transport mechanism termed transcytosis. To develop biotechnology-based drugs such as proteins, DNA, and siRNA as clinically effective therapeutics, transcytosis systems have been evaluated as a promising strategy to augment drug transfer across endothelial and epithelial barriers. Screening of random peptide libraries using phage display is a powerful technology to identify peptide sequences with high affinity for surface proteins on desired target cells. Here, we describe assembly of a diverse, cyclic heptapeptide library on the icosahedral T7 bacteriophage platform. This phage-displayed library of random peptides was used for functional in vitro screens across BeWo cell monolayers to identify peptide ligands that facilitate placental transcytosis of viral particles across this cell culture model of the human trophoblast barrier.
