Three genes control the timing, the site and the size of blastema formation in Drosophila.
Three genes control the timing, the site and the size of blastema formation in Drosophila.
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DOI：
10.1016/j.ydbio.2008.04.004
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发表时间：
2008-07
影响因子：
2.7
通讯作者：
K. McClure;Anne Sustar;G. Schubiger
中科院分区：
文献类型：
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作者：
K. McClure;Anne Sustar;G. Schubiger
再生是维持和修复组织的重要过程。尽管再生很重要，但负责再生生长的基因仍然很大程度上未知。在果蝇中，成虫盘再生可以通过片段化和体内培养诱导，也可以通过 wingless (wg/wnt1) 的普遍表达原位诱导。成虫盘就像低等脊椎动物的附肢一样，通过伤口愈合和伤口部位的增殖来启动再生，形成再生胚基。大多数胚基细胞在再生过程中保持其椎间盘特异性特征；然而，少数细胞表现出类似干细胞的特性并转变为不同的命运，这种现象称为转决定。我们鉴定了三个基因：再生 (rgn)、肝再生增强剂 (alr) 和基质金属蛋白酶-1 (Mmp1)，在椎间盘再生过程中在胚细胞中特异性表达。这些基因的突变通过延迟、减少或定位再生胚基来影响片段化和wg诱导的再生。除了胚基稳态的改变之外，这三个基因的突变还改变了再生诱导的转决定的速率。我们认为这些基因在再生增殖、生长和调节细胞可塑性中发挥作用。
Regeneration is a vital process to maintain and repair tissues. Despite the importance of regeneration, the genes responsible for regenerative growth remain largely unknown. In Drosophila, imaginal disc regeneration can be induced either by fragmentation and in vivo culture or in situ by ubiquitous expression of wingless (wg/wnt1). Imaginal discs, like appendages in lower vertebrates, initiate regeneration by wound healing and proliferation at the wound site, forming a regeneration blastema. Most blastema cells maintain their disc-specific identity during regeneration; a few cells however, exhibit stem-cell like properties and switch to a different fate, in a phenomenon known as transdetermination. We identified three genes, regeneration (rgn), augmenter of liver regeneration (alr) and Matrix metalloproteinase-1 (Mmp1) expressed specifically in blastema cells during disc regeneration. Mutations in these genes affect both fragmentation- and wg-induced regeneration by either delaying, reducing or positioning the regeneration blastema. In addition to the modifications of blastema homeostasis, mutations in the three genes alter the rate of regeneration-induced transdetermination. We propose that these genes function in regenerative proliferation, growth and regulate cellular plasticity.