Serum untargeted metabolomics delineates the metabolic status in different subtypes of non-alcoholic fatty liver disease
Serum untargeted metabolomics delineates the metabolic status in different subtypes of non-alcoholic fatty liver disease
复制标题
血清非靶向代谢组学描绘了非酒精性脂肪肝不同亚型的代谢状态
DOI：
10.1016/j.jpba.2021.114058
复制
发表时间：
2021-04-14
影响因子：
3.4
通讯作者：
Feng, Rennan
中科院分区：
文献类型：
--
作者：
Liu, Liyan;Zhao, Jinhui;Feng, Rennan
目的：本研究的目的是鉴定与非酒精性脂肪性肝病（NAFLD）相关的新的血清代谢物，并探讨瘦型NAFLD和肥胖型NAFLD之间的代谢差异。收集来自NAFLD患者（n = 161）和健康参与者（n = 149）的血清样品，采用UPLC-Q-TOF MS/MS分析代谢产物，并进行亚组分析，以探讨瘦型NAFLD、肥胖型NAFLD和健康对照者的代谢差异。在NAFLD患者和健康对照组之间共发现了24种差异存在的代谢物。在NAFLD亚型之间观察到显著的代谢途径差异，包括脂肪酸和氨基酸代谢。最终，五个代谢产物（普拉睾酮，吲哚硫酸，癸二酸，花生四烯酸和硫酸双烯醇酮）被用来建立一个诊断模型，以区分患者与NAFLD无论瘦或肥胖NAFLD type.Conclusions：这项研究表明，NAFLD亚型之间存在显着的代谢差异，我们的模型可能是有用的，以区分患者与NAFLD。这些发现可能为NAFLD亚型的检测和治疗奠定基础。(c)2021爱思唯尔有限公司版权所有。
Aims: The aim of this study was to identify novel serum metabolites associated with non-alcoholic fatty liver disease (NAFLD), and to explore the metabolic discrepancies between Lean-NAFLD and ObeseNAFLD.Methods: Serum samples from patients with NAFLD (n = 161) and healthy participants (n = 149) were collected, and metabolites were analyzed with UPLC-Q-TOF MS/MS. Subgroup analyses were performed to explore the metabolic differences among Lean-NAFLD, Obese-NAFLD and healthy controls Results: A total of 24 differentially present metabolites were found between patients with NAFLD and healthy controls. Marked metabolic pathway differences were observed among the NAFLD subtypes, including in fatty acid and amino acid metabolism. Ultimately, five metabolites (prasterone, indoxylsulfuric acid, sebacic acid, arachidonic acid and pregnenolone sulfate) were used to establish a diagnostic model to distinguish patients with NAFLD regardless of Lean-or Obese-NAFLD type.Conclusions: This study suggested that significant metabolic differences existed among subtypes of NAFLD, and our model might be useful to distinguish patients with NAFLD. These findings may lay a foundation for the detection and treatment of NAFLD subtypes.(c) 2021 Elsevier B.V. All rights reserved.