Variability in estimating eccentric contraction-induced muscle damage and inflammation in humans
Variability in estimating eccentric contraction-induced muscle damage and inflammation in humans
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DOI：
10.1139/h02-028
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发表时间：
2002-10-01
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影响因子：
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通讯作者：
Phillips, SM
中科院分区：
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作者：
Beaton, LJ;Tarnopolsky, MA;Phillips, SM
我们研究了5名年轻的健康志愿者，他们每隔一周对每条腿进行240次(24组x 10次/组)最大等速离心性肌肉收缩(30度/S)。两次取自股外侧肌的活检。根据损伤方案，从同一肌肉内取两次活组织检查24小时。第二次，按照相同的损伤方案，于24小时从对侧小腿取一次活组织检查。所有三个部位的活组织检查均显示Z带断裂，比静息活组织检查中通常观察到的大得多(类似于14倍)，根据部位的不同(腿内或腿间)没有显著差异(ANOVA)。然而，腿内变异系数(CV)为41+/-30%。腿间变异分别为57.36%和68+/-36%。在肌肉中也检测到巨噬细胞，不同生物部位的细胞数量没有统计学差异，但活检内的CV=52+/-19%，活检间的CV分别为34+/-24%和48+/-27%。我们的结论是，在人类肌肉活检样本中检测到的偏心收缩诱导的Z带流和炎性细胞反应是高度可变的，变异系数为40-70%。
We studied five young healthy volunteers who performed a "damage protocol" consisting of 240 (24 sets x 10 repetitions/set) maximal isokinetic eccentric muscle contractions (30degrees/s) on each leg one week apart. Biopsies were taken from the vastus lateralis on two occasions. Two biopsies were taken from within the same muscle 24h following the damage protocol. On a second occasion a single biopsy was taken from the contralateral leg at 24h following the same damage protocol. Biopsies at all three sites showed Z-band disruption, much greater (i.e., similar to14-fold) than is typically observed in resting biopsies, with no significant differences (ANOVA) according to site location (within legs or between legs). The within-leg coefficient of variation (CV) was, however, 41 +/- 30%. and the between-leg CVs were 57 36% and 68 +/- 36%. Macrophage cells were also detected within the muscle, and cell numbers were not statistically different between biopsysites, However, the within-biopsy CV = 52 +/- 19% and the between-biopsy CVs of 34 +/- 24% and 48 +/- 27%. We conclude that eccentric contraction-induced Z-band streaming and inflammatory cell response, as detected in muscle biopsy samples from humans, is highly variable with a CV of 40-70%.