Assessment of bovine cortical bone fracture behavior using impact microindentation as a surrogate of fracture toughness.
Assessment of bovine cortical bone fracture behavior using impact microindentation as a surrogate of fracture toughness.
复制标题
使用冲击微压痕作为断裂韧性的替代物来评估牛皮质骨骨折行为。
DOI：
10.1101/2023.08.07.552351
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Tang,SimonY
中科院分区：
文献类型：
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作者：
Jahani,Babak;Vaidya,Rachana;Jin,JamesM;Aboytes,DonaldA;Broz,KaitlynS;Khrotapalli,Siva;Pujari,Bhanuteja;Baig,WaleeM;Tang,SimonY
骨的断裂行为对于评价骨的力学性能和抗骨折能力至关重要。断裂韧性是一种内在的材料特性，它量化了材料在受控条件下承受裂纹扩展的能力。然而，正确进行断裂韧性测试需要获得校准的机械载荷框架和骨样本的破坏性测试，因此断裂韧性测试在临床上是不切实际的。冲击显微压痕模拟了断裂韧性测量的某些方面，但其与断裂韧性的关系仍然未知。在这项研究中，我们的目的是比较新鲜和煮牛骨的缺口断裂韧性和冲击显微压痕测量。制备骨成熟的牛骨标本（n= 48），其中一半煮沸以使有机基质变性，而另一半保持在冷冻条件下保存。所有样本均接受缺口断裂韧性测试，以确定其抗裂纹萌生（KIC），并使用OsteoProbe进行冲击微压痕测试，以获得骨材料强度指数（BMSi）。煮沸骨样品增加了变性胶原蛋白的含量，而矿物质密度和孔隙率不受影响。煮沸的骨也显示出KIC（P< .0001）和平均BMSi（P< .0001）的显著降低，导致骨对裂纹扩展的抵抗力受损。值得注意的是，平均BMSi与KIC具有高度相关性（r= 0.86;P< .001）。排序差异分析证实了2项措施之间的极好一致性。这项研究提供了第一个证据表明，冲击微压痕可以作为一个替代措施的骨折行为。冲击微压痕的潜力，以最小的样品破坏评估骨断裂阻力可以提供有价值的见解，骨健康，而不需要繁琐的测试设备和样品破坏。
The fracture behavior of bone is critically important for evaluating its mechanical competence and ability to resist fractures. Fracture toughness is an intrinsic material property that quantifies a material’s ability to withstand crack propagation under controlled conditions. However, properly conducting fracture toughness testing requires the access to calibrated mechanical load frames and the destructive testing of bone samples, and therefore fracture toughness tests are clinically impractical. Impact microindentation mimicks certain aspects of fracture toughness measurements, but its relationship with fracture toughness remains unknown. In this study, we aimed to compare measurements of notched fracture toughness and impact microindentation in fresh and boiled bovine bone. Skeletally mature bovine bone specimens (n= 48) were prepared, and half of them were boiled to denature the organic matrix, while the other half remained preserved in frozen conditions. All samples underwent a notched fracture toughness test to determine their resistance to crack initiation (KIC) and an impact microindentation test using the OsteoProbe to obtain the Bone Material Strength index (BMSi). Boiling the bone samples increased the denatured collagen content, while mineral density and porosity remained unaffected. The boiled bones also showed significant reduction in both KIC(P< .0001) and the average BMSi (P< .0001), leading to impaired resistance of bone to crack propagation. Remarkably, the average BMSi exhibited a high correlation with KIC(r= 0.86;P< .001). A ranked order difference analysis confirmed the excellent agreement between the 2 measures. This study provides the first evidence that impact microindentation could serve as a surrogate measure for bone fracture behavior. The potential of impact microindentation to assess bone fracture resistance with minimal sample disruption could offer valuable insights into bone health without the need for cumbersome testing equipment and sample destruction.