Lipopolysaccharide/endotoxin induces IL-18 via CD14 in human peripheral blood mononuclear cells in vitro
Lipopolysaccharide/endotoxin induces IL-18 via CD14 in human peripheral blood mononuclear cells in vitro
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DOI：
10.1006/cyto.2000.0783
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发表时间：
2000-12-01
期刊：
影响因子：
3.8
通讯作者：
Rossol, S
中科院分区：
文献类型：
--
作者：
Manigold, T;Bocker, U;Rossol, S
IL-18 与 IL-12 在生成 T 辅助细胞 1 细胞和细胞因子反应方面具有相同的活性。它通过IL-12独立的干扰素-γ合成介导LPS/内毒素致死性，并诱导细菌相关的器官衰竭，由于外周血单核细胞（PBMC）是IL-18的有效产生者，我们在体外研究了暴露于LPS和金黄色葡萄球菌（SAC）后IL-18的调节，新鲜分离的PBMC组成型表达IL-18 mRNA，在未刺激的预孵育后然而，48 小时后，IL-18 转录几乎无法通过 RT-PCR 检测到，但可以被 LPS 或 SAC 诱导（P
IL-18 shares activities with IL-12 in generating T-helper 1 cells and cytokine response. It mediates LPS/endotoxin lethality by IL-12 independent interferon-gamma synthesis and it induces bacteria-related organ failure, As peripheral blood mononuclear cells (PBMC) are potent producers of IL-18, we studied the regulation of IL-18 upon exposure to LPS and Staphylococcus aureus (SAC) in vitro, Freshly isolated PBMC constitutively expressed IL-18 mRNA, After unstimulated preincubation for 48 h, however, IL-18 transcripts mere nearly not detectable by RT-PCR, but inducible by LPS or SAC (P