Isolation of Actinomycetes from Pine Litter Layers.
Isolation of Actinomycetes from Pine Litter Layers.
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从松树凋落物层中分离放线菌。
DOI：
10.3209/saj.8_57
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
J. Watanabe
中科院分区：
文献类型：
--
作者：
S. Matsukuma;T. Okuda;J. Watanabe
对松树凋落物中放线菌的选择性分离方法进行了评价。在琼脂中加入0.05%的十二烷基硫酸钠溶液和添加20μg/ml的萘啶酸可有效地抑制细菌的生长。在琼脂中加入200μg/ml的苯磺酸盐能有效地抑制真菌的生长，用70%乙醇和1%氯化物次氯酸钠溶液表面灭菌的方法去除松针表面的放线菌，可以分离出寄生在松针蜡层下的放线菌。从松树枯枝落叶层分离得到的放线菌具有层次性和定量差异。
Methods for the selective isolation of actinomycetes from pine litter layers were evaluated. Pretreatment of source material with a 0.05% of sodium dodecyl sulfate solution and the addition of 20 μg/ml of nalidixic acid to the agar media were effective in reducing bacterial growth. The addition of 200 μg/ml of benlate to the agar medium was effective in reducing fungal growth.By removing actinomycetes from the surface of pine needles with the surface sterilization method by 70% of ethanol and sodium hypochlorite solution containing 1% of chloride, we were able to isolate those actinomycetes which reside under the waxy layer of needles.Comparative studies of isolates obtained from pine litter layers showed both a qualitative and quantitative difference by layers.