Molecular characterization, transcriptional regulation and association analysis with carcass traits of porcine TCAP gene.
Molecular characterization, transcriptional regulation and association analysis with carcass traits of porcine TCAP gene.
复制标题
DOI：
10.1016/j.gene.2014.01.043
复制
发表时间：
2014-04
期刊：
影响因子：
3.5
通讯作者：
M. Qiao;Jingshu Huang;Huayu Wu;Junjing Wu;Xianwen Peng;S. Mei
中科院分区：
文献类型：
--
作者：
M. Qiao;Jingshu Huang;Huayu Wu;Junjing Wu;Xianwen Peng;S. Mei
TCAP(又称titin-capp或telthonin)是一种与titin相互作用的Z盘蛋白，参与正常肌节结构的调节和发育。在本研究中，我们克隆了猪TCAP基因的全长编码区和启动子序列，其编码区长度为504hp。实时定量聚合酶链式反应(qRT-PCR)分析表明，PorcineTCAP在骨骼肌、心脏和肾脏中高表达。在出生后肌肉发育过程中，大白猪和梅山猪的TCAP表达从30天下调到120天。用等位基因特异性引物-聚合酶链式反应(ASP-PCR)检测了TCAP基因外显子2的单核苷酸多态性c.334G>；A。关联分析表明，该多态与部分屠宰性状存在显著关联(P<；0.05和P<；0.01)。在不同细胞系中对PorcineTCAP启动子的分析表明，它是一种肌肉特异性启动子。此外，我们还发现猪TCAP启动子可以被MyoD、MyoG和MEF2激活，这表明TCAP可能在猪骨骼肌发育过程中起着调节作用。这些发现为进一步研究TCAP在猪骨骼肌中的功能提供了有用的信息。
TCAP(also known as titin-cap or telethonin) is one of the titin interacting Z-disk proteins involved in the regulation and development of normal sarcomeric structure. In this study, we cloned the cDNA and promoter sequences of porcineTCAPgene, which contained a 504 bp full-length coding region. Quantitative real-time PCR (qRT-PCR) analyses showed that porcineTCAPwas highly expressed in the skeletal muscle, heart, and kidney. During postnatal muscle development,TCAPexpression was down-regulated from 30 days to 120 days in Large White and Meishan pigs. One single nucleotide polymorphism c.334G>A in exon 2 of theTCAPgene was identified and detected by allele-specific primer-polymerase chain reaction (ASP-PCR). Association analysis revealed that the polymorphism had significant associations (P< 0.05 andP< 0.01) with some carcass traits. Analysis of the porcineTCAPpromoter in different cell lines demonstrated that it is a muscle-specific promoter. In addition, we found that the porcineTCAPpromoter can be activated by MyoD, MyoG and MEF2 in myotubes, which indicated thatTCAPmay play a role in the regulation of porcine skeletal muscle development. These findings provide useful information for the further investigation of the function ofTCAPin porcine skeletal muscle.