Production of Nanoscale Vibration for Stimulation of Human Mesenchymal Stem Cells.
Production of Nanoscale Vibration for Stimulation of Human Mesenchymal Stem Cells.
复制标题
用于刺激人类间充质干细胞的纳米级振动的产生。
DOI：
10.1166/jbn.2016.2264
复制
发表时间：
2016
影响因子：
2.9
通讯作者：
Nikukar H
中科院分区：
文献类型：
--
作者：
Nikukar H
机械刺激正在成为生物工程中操纵细胞行为的常用技术，并在组织工程和可能的再生治疗中得到应用。活生物体在体内和体外对包括振动在内的各种机械刺激表现出生物反应。详细介绍了纳米级垂直运动装置的研制及其对小鼠内皮细胞（Le2）和人间充质干细胞（hMSCs）的影响。压电陶瓷促动器和铝增强器与激光干涉测量一起使用，以确保在细胞培养基质上的纳米级振幅一致性。在500和1000 Hz的频率下，施加在细胞上的峰值力估计为nN级。500 Hz刺激1周后，两种细胞类型的细胞骨架均发生形态学改变，MSC增殖增加。刺激后MSCs的核大小也发生了变化。
Mechanical stimulation is becoming a common technique for manipulating cell behaviour in bioengineering with applications in tissue engineering and possibly regenerative therapy. Living organisms show biological responses in vivo and in vitro to various types of mechanical stimulation including vibration. The development of apparatus to produce vertical motions of nanoscale amplitude is detailed and their effect on mouse endothelial (Le2) and human mesenchymal stem cells (hMSCs) is investigated. Piezo ceramic actuators and aluminium reinforcement were utilised along with laser interferometry to ensure amplitude consistency at the nanometre level across a cell culture substrate. Peak force applied to the cells was estimated to be of nN magnitude at frequencies of 500 and 1000 Hz. Morphological changes in the cytoskeleton were found for both cell types along with increased MSC proliferation after 1 week of stimulation at 500 Hz. Changes in the nuclear size of MSCs after stimulation were also found.