Dynamic Changes in the Localization of Neuronatin-Positive Cells during Neurogenesis in the Embryonic Rat Brain
Dynamic Changes in the Localization of Neuronatin-Positive Cells during Neurogenesis in the Embryonic Rat Brain
复制标题
胚胎大鼠脑神经发生过程中神经元阳性细胞定位的动态变化
DOI：
10.1159/000504359
复制
发表时间：
2019
影响因子：
2.7
通讯作者：
Kato Yukio
中科院分区：
文献类型：
--
作者：
Kanno Naoko;Fujiwara Ken;Yoshida Saishu;Kato Takako;Kato Yukio
Neuronatin（NNAT）是一种选择性表达于新生小鼠脑细胞质中的基因，参与新生小鼠神经发生。然而，NNAT在产前大脑发育中的特殊作用尚未确定，特别是在中期到晚期阶段。在这项研究中，我们进行了NNAT和SOX 2蛋白，核转录因子和神经干/祖细胞标记物，在胚胎天13.5，E16大鼠大脑的免疫组化分析。5，E20。5.在E13时，在发育中的大脑中广泛观察到NNAT信号。E20集中于终末下丘脑的前体和基底部、丘脑2号前体的后板以及侧脑室和第四脑室的脉络丛。5.特别是，乳头体的底部的终端下丘脑，一个区域与大量的SOX 2阳性细胞，证明了强烈的NNAT信号E20。5. NNAT的细胞内定位表现出不同的配置文件，表明NNAT参与各种细胞功能，如细胞分化和功能的维护，在产前神经发生在大鼠脑。因此，目前的观察表明，NNAT蛋白在神经发生中的多样和积极的作用。确定这种分子的功能可能有助于阐明参与大脑发育的机制。
Neuronatin (NNAT) was first identified as a gene selectively and abundantly expressed in the cytoplasm of the newborn mouse brain, and involved in neonatal neurogenesis. However, the particular roles of NNAT in the developing prenatal brain have not been identified, especially in mid to late stages. In this study, we performed immunohistochemical analyses of NNAT and SOX2 proteins, a nuclear transcription factor and neural stem/progenitor marker, in the rat brain on embryonic days 13.5, E16. 5, and E20. 5. NNAT signals were broadly observed across the developing brain on E13. 5 and gradually more localized in later stages, eventually concentrated in the alar and basal parts of the terminal hypothalamus, the alar plate of prosomere 2 of the thalamus, and the choroid plexus in the lateral and fourth ventricles on E20. 5. In particular, the mammillary body in the basal part of the terminal hypothalamus, a region with a high number of SOX2-positive cells, evidenced intense NNAT signals on E20. 5. The intracellular localization of NNAT showed diverse profiles, suggesting that NNAT was involved in various cellular functions, such as cell differentiation and functional maintenance, during prenatal neurogenesis in the rat brain. Thus, the present observations suggested diverse and active roles of the NNAT protein in neurogenesis. Determining the function of this molecule may assist in the elucidation of the mechanisms involved in brain development.