A LIGHT AND ELECTRON-MICROSCOPIC LEVEL ANALYSIS OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) IN THE SPINAL-CORD OF THE PRIMATE - AN IMMUNOHISTOCHEMICAL STUDY
A LIGHT AND ELECTRON-MICROSCOPIC LEVEL ANALYSIS OF CALCITONIN GENE-RELATED PEPTIDE (CGRP) IN THE SPINAL-CORD OF THE PRIMATE - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1016/0304-3940(87)90119-4
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发表时间：
1987-11-23
影响因子：
2.5
通讯作者：
COGGESHALL, RE
中科院分区：
文献类型：
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作者：
CARLTON, SM;MCNEILL, DL;COGGESHALL, RE
在本研究中，降钙素基因相关肽（CGRP）染色的纤维和静脉曲张的分布在光学显微镜水平上在猴子的腰脊髓中得到了证明。 I、IIo 层、V 层网状区域和中央管区域的致密神经丛的免疫染色纤维和静脉曲张。曲张间纤维的直径始终测量为1μm或更小，这表明有细髓鞘或无髓鞘纤维的群体。在电子显微镜水平上，两种类型的末端被标记：一种是肾小球型，其中一个 CGRP 标记的轮廓被几个未标记的突触后轮廓缩进；另一种是轴突型，具有一个或有时两个突触后元件。 CGRP 标记谱最值得注意的细胞学特征是存在许多相对较大的具有致密核心的囊泡。这些发现有助于理解猴子背角中 CGRP 的突触相互作用。
In the present study, the distribution of calcitonin gene-related peptide (CGRP)-stained fibers and varicosities are demonstrated in the lumbar spinal cord of the monkey at the light microscopic level. Immunostained fibers and varicosities from a dense plexus in laminae I, IIo, the reticulated region of lamina V, and in the region of the central canal. The intervaricose fibers consistently measured 1 .mu.m or less in diameter suggesting a population of finely myelinated or unmyelinated fibers. At the electron microscopic level, two types of terminals were labeled: a glomerular type where one CGRP-labeled profiles was indented by several unlabeled postsynaptic profiles and an axodendritic type with one or sometimes two postsynaptic elements. The most noteworthy cytologic feature of CGRP-labeled profiles was the presence of many relatively large vesicles with dense cores. These findings are steps towards understanding the synaptic interactions of CGRP in the monkey dorsal horn.