Temperature-pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly(N-isopropylacrylamide-co-acrylic acid)
Temperature-pH sensitivity of bovine serum albumin protein-microgels based on cross-linked poly(N-isopropylacrylamide-co-acrylic acid)
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DOI：
10.1016/j.colsurfb.2006.03.020
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发表时间：
2006-06-01
影响因子：
5.8
通讯作者：
Kobayashi, T
中科院分区：
文献类型：
--
作者：
Huo, DX;Li, YN;Kobayashi, T
采用无皂乳液聚合法合成了不同丙烯酸（AAc）含量和交联剂NAP-亚甲基双丙烯酰胺（MBAAm）含量的单分散聚（N-异丙基丙烯酰胺-co-丙烯酸）（PNIPAM/AAc）微凝胶。随着AAc含量的增加，微凝胶对牛血清白蛋白（BSA）的结合能力增强。BSA的吸附量强烈依赖于悬浮液的pH值。在pH 4.0左右观察到最大吸附，而低pH和碱性条件诱导相当低的吸附。此外，AFM图像显示，微凝胶粒子通过负载BSA发生桥接聚集。负载BSA后，所得BSA-微凝胶颗粒变得更加疏水。重要的是，发现BSA吸附到PNIPAM/AAc微凝胶上改变所得微凝胶悬浮液的溶胀/去溶胀行为。随着BSA浓度的增加，BSA-微凝胶颗粒的体积相变温度向低温方向移动。(C)2006 Elsevier B. V.保留所有权利。
Monodispersed poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM/AAc) microgels with various contents of acrylic acid (AAc) and crosslinker NAP-methylenebisacrylamide (MBAAm) were synthesized by surfactant-free emulsion polymerization. The microgels exhibited affinity of binding bovine serum albumin (BSA) with increase of AAc contents. The adsorption amount of BSA was strongly dependent on suspension pH. The maximum adsorption was observed at around pH 4.0, while both low pH and basic conditions induced the rather low adsorption. In addition, AFM images showed that the microgel particles underwent bridging aggregation by loading BSA. The resultant BSA-microgel particles became more hydrophobic after loading BSA. Importantly, the adsorption of BSA onto PNIPAM/AAc microgel was found to alter the swelling/deswelling behavior of the resultant microgel suspensions. With the increase of BSA concentration, the volume phase transition temperature of BSA-microgel particles shifted to the lower temperature. (C) 2006 Elsevier B.V. All rights reserved.