Derepression of ferritin messenger RNA translation by hemin in vitro.
Derepression of ferritin messenger RNA translation by hemin in vitro.
复制标题
体外氯化血红素对铁蛋白信使 RNA 翻译的抑制。
DOI：
10.1126/science.2294594
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Thach,RE
中科院分区：
文献类型：
--
作者：
Lin,JJ;Daniels-McQueen,S;Patino,MM;Gaffield,L;Walden,WE;Thach,RE
将90千吨的铁蛋白抑制蛋白（FRP）与hemin或Co3+-原卟啉IX孵育，无论是游离的还是与l -铁蛋白转录物络合的，都可以防止随后在小麦胚芽提取物中抑制铁蛋白的合成。FeCl3in与H2O2的结合、Fe3+或Fe2+与EDTA的螯合、Zn2+-原卟啉IX和原卟啉IX均未引起玻璃钢的显著失活。sds -聚丙烯酰胺凝胶电泳显示，经hemin灭活的FRP在化学上保持完整。包括铁螯合剂或自由基清除剂没有改变血红素产生的失活。这些结果和其他结果表明，血红素在体外抑制铁蛋白的合成。
Incubation of a 90-kilodalton ferritin repressor protein (FRP), either free or complexed with an L-ferritin transcript, with hemin or Co3+-protoporphyrin IX prevented subsequent repression of ferritin synthesis in a wheat germ extract. Neither FeCl3in combinations with H2O2, nor Fe3+or Fe2+chelated with EDTA, nor Zn2+-protoporphyrin IX, nor protoporphyrin IX caused significant inactivation of FRP. FRP that had been inactivated by hemin remained chemically intact, as revealed by SDS-polyacrylamide gel electrophoresis. Inclusion of chelators of iron or free radical scavengers did not alter the inactivation produced by hemin. These and other results indicate that hemin derepresses ferritin synthesis in vitro.