Differential expression of S1 and elongation factor-1 alpha during rat development.
Differential expression of S1 and elongation factor-1 alpha during rat development.
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发表时间：
1993-11
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通讯作者：
S. Lee;L. Wolfraim;E. Wang
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作者：
S. Lee;L. Wolfraim;E. Wang
延伸因子-1 α (EF-1 α)是一种高度保守的蛋白，在翻译过程中参与肽的延伸。分离到cDNA S1；推导出的氨基酸序列与哺乳动物EF-1 α - s具有较高的相似性（92%）。EF-1 α存在于所有组织中，S1 mRNA只能在脑、心脏和肌肉中检测到。我们在此报告，逆行伪基因现象可归因于EF-1 α而不是S1，后者在大鼠基因组中由单个拷贝表示。S1阳性组织中S1稳态mRNA水平始终高于EF-1 α。S1 mRNA只能在体内大脑、心脏和肌肉发育后期检测到，并在出生后早期达到平台期。在培养肌肉系统中，S1的表达依赖于肌管的形成，尽管S1 mRNA的积累明显低于在成人骨骼肌中观察到的。EF-1 α mRNA水平在大脑、心脏和肌肉发育过程中下调，但在肝脏中保持相对稳定。我们在这里发现EF-1 α和S1在大鼠发育过程中存在差异表达，S1基因表达的激活是在脑、心脏和肌肉的终末分化过程之后发生的。
Elongation factor-1 alpha (EF-1 alpha) is a highly conserved protein functioning in peptide elongation during translation. A cDNA, S1, was isolated; its deduced amino acid sequence shares high similarity with mammalian EF-1 alpha s (92%). While EF-1 alpha is present in all tissues, S1 mRNA can only be detected in brain, heart, and muscle. We report here that the retropseudogene phenomenon is attributable to EF-1 alpha and not S1, the latter being represented by a single copy in the rat genome. The S1 steady-state mRNA levels are consistently higher than EF-1 alpha in S1-positive tissues. S1 mRNA can only be detected late during brain, heart, and muscle development in vivo and increases to a plateau in early postnatal life. In a cultured muscle system, S1 expression is dependent upon the formation of myotubes, although the accumulation of S1 mRNA is significantly lower than that observed in adult skeletal muscle. EF-1 alpha mRNA levels are down-regulated during brain, heart, and muscle development, but stay relatively steady in liver. We show here that EF-1 alpha and S1 are differentially expressed during rat development and that the activation of S1 gene expression is subsequent to the terminal differentiation process in brain, heart, and muscle.