Pumilicin 4, a novel bacteriocin with anti-MRSA and anti-VRE activity produced by newly isolated bacteria Bacillus pumilus strain WAPB4
Pumilicin 4, a novel bacteriocin with anti-MRSA and anti-VRE activity produced by newly isolated bacteria Bacillus pumilus strain WAPB4
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DOI：
10.1007/s00284-006-0632-2
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发表时间：
2007-10-01
影响因子：
2.6
通讯作者：
Na-Bangchang, Keasara
中科院分区：
文献类型：
--
作者：
Aunpad, Ratchaneewan;Na-Bangchang, Keasara
从泰国4个地区采集的69份土壤和水样中分离出34株具有抗耐甲氧西林金黄色葡萄球菌（MRSA）活性的细菌。一株被鉴定为短小芽孢杆菌的菌株WAPB 4对MRSA、耐万古霉素粪肠球菌（VRE）和几种革兰氏阳性试验菌显示出显著的抗菌活性。WAPB 4产生的细菌素被命名为pumilicin 4。它在121 ℃下热稳定15分钟，在pH 3-9范围内有活性。当用链霉蛋白酶E、胰凝乳蛋白酶和胰蛋白酶处理时，其活性消失，表明其蛋白质性质。在高剂量（80 Au mL（-1））下，pumilicin 4对MRSA和VRE均具有杀菌作用。在低剂量细菌素（20 Au mL（-1））下观察到抑菌作用。通过三步程序进行短小霉素4的纯化，即，溶剂萃取、固相萃取和反相色谱法。通过质谱法测定纯化的朴霉素4的分子量为1994.62道尔顿。本研究首次报道了由B产生的一种新型细菌素--pumilicin 4。具有用作治疗MRSA和VRE感染的替代抗菌剂的潜力。
A total of 34 bacterial strains with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity were isolated from 69 soil and water samples collected from four areas of Thailand. One strain, WAPB4 identified as Bacillus pumilus, showed remarkable antibacterial activity against MRSA, vancomycin-resistant Enterococcus faecalis (VRE), and several Gram-positive test bacteria. Bacteriocin produced by WAPB4 was designated as pumilicin 4. It was heat stable up to 121 degrees C, 15 min and active within the pH range of 3-9. Its activity disappeared when treated with pronase E, chymotrypsin, and trypsin, demonstrating its proteinaceous nature. At high dosage (80 AU mL(-1)), the effect of pumilicin 4 was bactericidal to both MRSA and VRE. Bacteriostasis was observed for a low dose of bacteriocin (20 AU mL(-1)). Purification of pumilicin 4 was performed by a three-step procedure, i.e., solvent extraction, solid phase extraction, and reversed-phase chromatography. The molecular mass of purified pumilicin 4 as determined by mass spectrometry was 1994.62 Dalton. This present study is the first report of a novel bacteriocin, pumilicin 4, produced by B. pumilus that has potential for use as an alternative antibacterial agent for the treatment of infection with MRSA and VRE.