Genome Analysis of Programmed DNA Elimination in Parasitic Nematodes.
Genome Analysis of Programmed DNA Elimination in Parasitic Nematodes.
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DOI：
10.1007/978-1-0716-1681-9_14
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发表时间：
2021
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两种不同的寄生线虫使用程序性DNA消除来沉默体细胞中的生殖系表达基因（蛔虫）或用于性别决定（类圆线虫属）。在蛔虫中，DNA在早期胚胎发生期间仅在前体细胞中丢失，导致与完整生殖细胞基因组相比减少的体细胞基因组。比较基因组分析提供的信息保留与消除序列，DNA断裂，一个完整的染色体观点的DNA消除，和进化保守的DNA消除蛔虫。这些研究揭示了DNA消除的功能和机制的新见解，为深入研究DNA消除的分子机制提供了参考。在这里，我描述了我们用来研究程序化DNA消除的基因组学方法，重点是寄生线虫蛔虫。
Two distinct groups of parasitic nematodes use programmed DNA elimination to silence germline-expressed genes in the somatic cells (ascarids) or for sex determination (Strongyloides spp.). In the ascarids, DNA is lost only in pre-somatic cells during early embryogenesis, leading to a reduced somatic genome compared to the intact germ cell genome. Comparative genome analysis has provided information on the retained vs. eliminated sequences, DNA breaks, a full chromosome view on DNA elimination, and the evolutionary conservation of DNA elimination among ascarids. These studies have revealed novel insights into the functions and mechanisms of DNA elimination and provided a reference for in-depth molecular analysis of DNA elimination. Here, I describe the genomics methods we used to study programmed DNA elimination, focusing on the parasitic nematode Ascaris.