Sugar-regulated expression of a putative hexose transport gene in grape
Sugar-regulated expression of a putative hexose transport gene in grape
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受糖调节的葡萄假定己糖转运基因的表达
DOI：
10.1104/pp.009522
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发表时间：
2003-01-01
期刊：
影响因子：
7.4
通讯作者：
Delrot, S
中科院分区：
文献类型：
--
作者：
Atanassova, R;Leterrier, M;Delrot, S
葡萄启动子（Vitis Vinifera）VVHT1（Hexose Transporter 1）基因的不同长度，该基因编码在葡萄成熟期间表达的假定己糖转运蛋白，已转录到β-葡萄糖醛酸酶报告基因。在用这些构建体转化的转基因烟草（烟草Tabacum）中，VVHT1启动子显然负责接收器官的优先表达。研究了VVHT1启动子的潜在糖效应子在用嵌合构建体转化的烟草CV亮黄色的2个细胞中。葡萄糖（56毫米），蔗糖（SUC; 58 mm）和非传输的SUC ISOMAR PALATINOSE将VVHT1启动子赋予的β-葡萄糖醛酸酶活性加倍，而果糖并不影响它。这些作用是2.4-kb启动子最强的效果，其中包含所有假定的糖响应元件（激活和抑制），但它们对0.3-kb启动子也很重要，而0.3-kb启动子仅包含激活的糖盒。在同源的葡萄浆果细胞培养中证实了SUC和palatinose诱导VVHT1表达。数据提供了假定的糖转运蛋白的第一个例子，该糖转运蛋白是由葡萄糖和较高植物中的SUC诱导的。尽管通过SUC诱导VVHT1表达并不需要转运，但葡萄糖基部分的存在对于SUC感应是必要的。这些结果为植物中的糖传感和信号传导提供了新的见解。
Different lengths of the promoter of grape (Vitis vinifera) VvHT1 (Hexose Transporter 1) gene, which encodes a putative hexose transporter expressed during the ripening of grape, have been transcriptionally fused to the beta-glucuronidase reporter gene. In transgenic tobacco (Nicotiana tabacum) transformed with these constructs, VvHT1 promoters were clearly responsible for the sink organ preferential expression. The potential sugar effectors of VvHT1 promoter were studied in tobacco cv Bright-Yellow 2 cells transformed with chimeric constructs. Glucose (56 mM), sucrose (Suc; 58 mM), and the non-transported Suc isomer palatinose doubled the beta-glucuronidase activity conferred by the VvHT1 promoter, whereas fructose did not affect it. These effects were the strongest with the 2.4-kb promoter, which contains all putative sugar-responsive elements (activating and repressing), but they were also significant with the 0.3-kb promoter, which contains only activating sugar boxes. The induction of VvHT1 expression by both Suc and palatinose was confirmed in the homologous grape berry cell culture. The data provide the first example of a putative sugar transporter, which is induced by both glucose and Suc in higher plants. Although induction of VvHT1 expression by Suc does not require transport, the presence of glucosyl moiety is necessary for Suc sensing. These results provide new insights into sugar sensing and signaling in plants.