Allelopathic effects of the extracts from an invasive species Solidago canadensis L. on Microcystis aeruginosa
Allelopathic effects of the extracts from an invasive species Solidago canadensis L. on Microcystis aeruginosa
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入侵物种加拿大一枝黄花提取物对铜绿微囊藻的化感作用
DOI：
10.1111/lam.12133
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发表时间：
2013-11-01
影响因子：
2.4
通讯作者：
Kong, H.
中科院分区：
文献类型：
--
作者：
Huang, Y.;Bai, Y.;Kong, H.
本研究调查了加拿大一枝黄花（Solidago canadensis L.）对铜绿微囊藻（Microcystis aeruginosa）的化感作用。结果表明，加拿大一枝黄花提取物能显著抑制铜绿微囊藻的生长。0.3和0.5克/升提取物的样品在7天后抑制率超过90%，透射电子显微镜图像显示在培养过程中铜绿微囊藻细胞受到损伤。在生理生化测定中，随着活性氧（ROS）的积累，膜通透性和丙二醛（MDA）含量迅速增加，抗氧化分子（抗坏血酸（AsA）和谷胱甘肽（GSH））含量增加。尽管在低提取物浓度下抗氧化酶（超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT））活性增加，但当提取物浓度增加时则表现为抑制作用。总之，本研究为利用有害杂草加拿大一枝黄花控制有害蓝藻提供了新思路。铜绿微囊藻细胞生理生化的改变不是孤立的，而是随着细胞内活性氧的刺激，活性氧在加拿大一枝黄花提取物的抑制作用中可能起关键作用。据推测，陆生植物可能具有与水生植物相同的抑藻机制。
This study investigated allelopathic effects of Solidago canadensis L. on Microcystis aeruginosa. The results showed that S.canadensis L. extracts could significantly inhibit the growth of M.aeruginosa. The inhibition ratios of samples with 03 and 05gl(-1) extracts were over 90% after 7days, and the transmission electron microscopy images showed the damage of M.aeruginosa cells during the incubation. In physiological and biochemical measurements, the membrane permeability and malondialdehyde (MDA) content rapidly increased with the accumulation of reactive oxygen species (ROS), and the content of antioxidant molecules (ascorbic acid (AsA) and glutathione (GSH)) increased. Although the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)) increased at low extracts concentrations, the effects were inhibitory when the extracts concentration increased. In conclusion, this study provided a new idea to utilize the detrimental weed S.canadensis L. to control harmful cyanobacteria. The alterations in physiology and biochemistry of M.aeruginosa cell were not in isolation, but with the stimulation of intracellular ROS that could play a fundamental role in inhibitory effects of S.canadensis L. extracts. It was inferred that terrestrial plants could have the same algistatic mechanisms as hydrophytes.