Functional characterization of FT and MFT ortholog genes in orchid (Dendrobium nobile Lindl) that regulate the vegetative to reproductive transition in Arabidopsis
Functional characterization of FT and MFT ortholog genes in orchid (Dendrobium nobile Lindl) that regulate the vegetative to reproductive transition in Arabidopsis
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DOI：
10.1007/s11240-012-0178-x
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发表时间：
2012-11-01
影响因子：
3
通讯作者：
Li, Hongqing
中科院分区：
文献类型：
--
作者：
Li, Ruihong;Wang, Aike;Li, Hongqing
磷酸乙醇胺结合蛋白(PEBP)超家族基因在高等植物营养生长和生殖生长之间的转换中起着重要的作用。在此，我们分离到了与开花基因T(FT)和开花基因母(MFT)同源的金银花peBP超家族的两个基因。这两个基因分别命名为DnFT和DnMFT，主要在植物的副芽和叶片中表达。在副芽中，DnFT在幼芽中的表达水平高于成熟芽，而成熟叶中DnFT的表达水平高于年轻叶。低温处理导致DnFT在叶片中的表达增加，而在芽中的表达降低。而在花芽发育过程中，DnMFT在芽中的表达增加，在叶片中的表达降低，且不受低温处理的影响。DnFT在拟南芥中的异位表达表现为早花表型和花序不确定性丢失。DnMFT在拟南芥中的过度表达导致了略微延迟的开花。我们的结果表明，DnFT通过与其在拟南芥中的同源基因相似的方式来调控花的转换，从而在金银花中作为花的诱导剂发挥作用。低温诱导的早花可能是由于DnFT转录被激活所致。
Genes belonging to the phosphoethanolamine binding protein (PEBP) superfamily play important roles in controlling the switch between vegetative and reproductive growth in higher plants. Here we reported the isolation of two genes from the PEBP superfamily in Dendrobium nobile Lindl homologous to FLOWERING LOCUS T (FT) and MOTHER OF FT (MFT). These two genes, designated as DnFT and DnMFT, were predominantly expressed in the auxiliary buds and leaves of the plant. In auxiliary buds, DnFT was expressed at a higher level in young buds than in mature buds, while mature leaves expressed more DnFT than young ones. Low temperature treatment led to an increased expression of DnFT in leaves, but a decreased expression in buds. In contrast, the expression of DnMFT increased in buds and decreased in leaves during flower bud development and was not influenced by low temperature treatment. Ectopic expression of DnFT in Arabidopsis plants showed an early-flowering phenotype and inflorescence indeterminacy loss. Over-expression of DnMFT in Arabidopsis led to slightly late-flowering. Our results revealed that DnFT functions as a floral inducer in D. nobile Lindl by regulating flower transition in a similar way to its ortholog in Arabidopsis. Early flowering, induced by a low temperature treatment, was probably due to the activation of DnFT transcription in D. nobile Lindl.