Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica.
Sialic acid-dependent attachment of mucins from three mouse strains to Entamoeba histolytica.
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DOI：
10.1016/j.bbrc.2013.05.085
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发表时间：
2013-06-28
影响因子：
3.1
通讯作者：
Shinohara, Yasuro
中科院分区：
文献类型：
--
作者：
Kato, Kentaro;Takegawa, Yasuhiro;Ralston, Katherine S.;Gilchrist, Carol A.;Hamano, Shinjiro;Petri, William A., Jr.;Shinohara, Yasuro
小鼠不同品系的肠道粘蛋白碳水化合物组成的差异被假设为解释了品系依赖的对溶组织内阿米巴的易感性。为了验证这一假设，分析了来自敏感和抗性近交系小鼠的肠道粘蛋白的O-糖蛋白含量以及它们抑制阿米巴与(GalNAc)12-27-HSA新糖蛋白黏附的能力。结果表明，易感CBA小鼠大肠粘蛋白O-葡聚糖的唾液酸含量低于耐药C57BL/6和BALB/c小鼠。CBA小鼠的粘蛋白比C57BL/6或BALB/c小鼠的粘蛋白更能抑制溶组织内阿米巴与新糖蛋白的黏附。与末端Gal/GalNAc作为阿米巴黏附受体的作用一致，唾液酸酶处理C57BL/6和BALB/c结直肠粘蛋白增强了它们抑制溶组织性肠杆菌对新糖蛋白黏附的能力。这些结果提供了小鼠品系特异性粘蛋白O-糖链唾液酸含量差异的证据。这些差异可能有助于这三个小鼠品系对溶组织内阿米巴感染的不同敏感性。
Mouse strain-specific differences in the carbohydrate composition of intestinal mucins were hypothesized to account for strain-dependent susceptibility to Entamoeba histolytica. To test this hypothesis, intestinal mucins from susceptible and resistant inbred strains of mice were analyzed for their O-glycan content and for their ability to inhibit amoebic adherence to (GalNAc)12-27-HSA neo-glycoproteins. The results showed that the colorectal mucin O-glycan of susceptible CBA mice was lower in sialic acid content than that of resistant C57BL/6 and BALB/c mice. Mucins from CBA mice were more potent inhibitors of Entamoeba histolytica adherence to neo-glycoproteins than were mucins from C57BL/6 or BALB/c mice. Consistent with the role of terminal Gal/GalNAc as a receptor for amoebic adherence, sialidase treatment of C57BL/6 and BALB/c colorectal mucins increased their ability to inhibit E. histolytica adherence to the neo-glycoproteins. These results provide evidence of mouse strain-specific differences in the sialic acids content of mucin O-glycans. These dissimilarities likely contribute to the differential susceptibility of the three mouse strains to Entamoeba histolytica infection.
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DOI：
10.1513/pats.2306016
发表时间：
2004-01-01
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Thornton, David J;Sheehan, John K
通讯作者：
Sheehan, John K
影响因子：
4.8
作者：
ADLER, P;WOOD, SJ;SCHNAAR, RL
通讯作者：
SCHNAAR, RL
影响因子：
7.4
作者：
Matsuno, Yu-ki;Saito, Takuro;Kameyama, Akihiko
通讯作者：
Kameyama, Akihiko
影响因子：
3.1
作者：
Asgharpour, A;Gilchrist, C;Houpt, E
通讯作者：
Houpt, E
影响因子：
4.3
作者：
Larsson, Jessica M. Holmen;Karlsson, Hasse;Hansson, Gunnar C.
通讯作者：
Hansson, Gunnar C.