Global increase in DNA methylation during orange fruit development and ripening
Global increase in DNA methylation during orange fruit development and ripening
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DOI：
10.1073/pnas.1815441116
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发表时间：
2019-01-22
影响因子：
11.1
通讯作者：
Lang, Zhaobo
中科院分区：
文献类型：
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作者：
Huang, Huan;Liu, Ruie;Lang, Zhaobo
DNA甲基化是参与许多生物过程的重要表观遗传标记。由于在成熟过程中活跃的DNA去甲基化，更年期番茄果实的基因组经历了DNA甲基化的全球损失。目前尚不清楚其他水果的成熟是否也与整体DNA去甲基化有关。我们对甜橙果实的单碱基分辨率DNA甲基组进行了表征。与未成熟的橙子相比，成熟的橙子在超过30000个基因组区域获得DNA甲基化，而在大约1000个基因组区域丢失DNA甲基化，这表明在橙子成熟过程中，DNA甲基化在全球范围内增加。DNA甲基化的增加与DNA去甲基化酶基因表达的减少有关。DNA甲基化抑制剂的应用干扰了成熟，表明DNA超甲基化对橙子果实的正常成熟至关重要。我们发现，成熟相关的DNA超甲基化与数百个基因的抑制（如光合作用基因）和数百个基因的激活（包括参与脱落酸反应的基因）有关。我们的研究结果表明DNA甲基化在橙子果实成熟过程中起重要作用。
DNA methylation is an important epigenetic mark involved in many biological processes. The genome of the climacteric tomato fruit undergoes a global loss of DNA methylation due to active DNA demethylation during the ripening process. It is unclear whether the ripening of other fruits is also associated with global DNA demethylation. We characterized the single-base resolution DNA methylomes of sweet orange fruits. Compared with immature orange fruits, ripe orange fruits gained DNA methylation at over 30,000 genomic regions and lost DNA methylation at about 1,000 genomic regions, suggesting a global increase in DNA methylation during orange fruit ripening. This increase in DNA methylation was correlated with decreased expression of DNA demethylase genes. The application of a DNA methylation inhibitor interfered with ripening, indicating that the DNA hypermethylation is critical for the proper ripening of orange fruits. We found that ripening-associated DNA hypermethylation was associated with the repression of several hundred genes, such as photosynthesis genes, and with the activation of hundreds of genes, including genes involved in abscisic acid responses. Our results suggest important roles of DNA methylation in orange fruit ripening.