Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor
Engineering of an elastic large muscular vessel wall with pulsatile stimulation in bioreactor
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在生物反应器中进行脉动刺激的弹性大肌肉血管壁工程。
DOI：
10.1016/j.biomaterials.2007.11.037
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发表时间：
2008-04-01
期刊：
影响因子：
14
通讯作者：
Cao, Yilin
中科院分区：
文献类型：
--
作者：
Xu, Zhi C.;Zhang, Wen J.;Cao, Yilin
组织工程为体外构建具有适当机械性能的血管替代品提供了一种新方法。尽管在小血管（直径< 6毫米）的工程化方面取得了成功，但由于其生物力学性能不足，工程化大血管（直径> 6毫米）仍然是一个挑战。在当前的研究中，通过在动态培养条件设计的容器反应器上装载接种了平滑肌细胞（SMC）的聚乙醇酸（PGA）无纺纤维支架来设计弹性大血管壁（直径6朗姆）。从犬颈动脉中分离出 SMC，并在接种到 PGA 纤维网上之前进行扩增。然后将细胞接种的 PGA 网加载到容器反应器上并接受脉动刺激。经过8周的动态工程，大体上形成了弹性血管壁。组织学检查显示动态培养组平滑肌细胞和胶原纤维定向良好。此外，平滑肌α-肌动蛋白和钙调蛋白的阳性染色证实了SMC的表型。相反，在无刺激的静态培养下，观察到平滑肌细胞和胶原纤维紊乱。此外，与静态培养的工程血管相比，动态培养的工程血管在生物力学性能上表现出显着的改善。我们的结果表明，当前工作中开发的方法对于大型船舶工程来说是有效的。这种方法也可能适用于具有肌肉管状结构的其他组织的工程。 (c) 2007 Elsevier Ltd. 保留所有权利。
Tissue engineering offers a new approach for the construction of vascular substitutes in vitro with proper mechanical properties. Although success has been made in the engineering of small blood vessels (< 6 mm in diameter), it remains a challenge to engineer large vessels (> 6 mm in diameter) due to their insufficient biomechanical property. In the current study, an elastic large vessel wall (6 rum in diameter) was engineered by loading a polyglycolic acid (PGA) unwoven fiber scaffold seeded with smooth muscle cells (SMCs) on a vessel reactor designed with dynamic culture conditions. SMCs were isolated from canine carotid artery and expanded before seeding on a PGA fiber mesh. The cell-seeded PGA mesh was then loaded on a vessel reactor and subjected to pulsatile stimuli. Grossly, an elastic vessel wall was formed after 8 weeks of dynamic engineering. Histological examination showed well-orientated smooth muscle cells and collagenous fibers in the group with dynamic culture. In addition, the phenotype of SMCs was confirmed by positive staining of smooth muscle alpha-actin and calponin. On the contrary, disorganized smooth muscle cells and collagenous fibers were observed in the group under static culture without stimuli. Furthermore, the engineered vessels under dynamic culture exhibited significant improvements on biomechanical property over the one from static culture. Our results indicate that the approach developed in the current work is efficient for large vessel engineering. This approach may also be suitable for the engineering of other tissues with muscular tubular structure. (c) 2007 Elsevier Ltd. All rights reserved.