Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes
Mechanism of vitamin D3-induced transcription of phospholipase D1 in HaCat human keratinocytes
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DOI：
10.1016/j.febslet.2007.03.073
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发表时间：
2007-05-01
期刊：
影响因子：
3.5
通讯作者：
Murate, Takashi
中科院分区：
文献类型：
--
作者：
Kikuchi, Ryosuke;Sobue, Sayaka;Murate, Takashi
在HaCaT人角质形成细胞中，1 α，25-二羟基维生素D-3（VitD（3））增加磷脂酶DI（PLD 1）的蛋白质和基因表达，但不增加PLD 2。我们发现，维生素D3通过5'PLD 1启动子上的维生素D反应元件（VDRE）（外显子1的-260到-246）增加PLD 1基因表达。通过将VitD 3受体（VDR）转染到HEK 293细胞中获得了类似的结果，该细胞最初对VitD（3）无反应。电泳迁移率变动分析（EMSA）和染色质免疫沉淀（CHIP）分析表明，VitD 3、VDR和维甲酸X受体α（RXR α）的复合物与VDRE结合，并增加HaCaT细胞中PLD 1基因的表达。(C)2007年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
1 alpha,25-Dihydroxyvitamin D-3 (VitD(3)) increases protein and gene expression of phospholipase DI (PLD1), but not PLD2, in HaCaT human keratinocytes. We show that VitD3 increases PLD1 gene expression through a vitamin D responsive element (VDRE) on the 5' PLD1 promoter (-260 by to -246 by from exon 1). Similar results were obtained by transfecting VitD3 receptor (VDR) into HEK293 cells, which are originally VitD(3)-unresponsive. Electrophoresis mobility shift assays (EMSA) and chromatin immunoprecipitation (CHIP) assays showed that the complex of VitD3, VDR and retinoid X receptor a (RXR alpha) binds to the VDRE and increases PLD1 gene expression in HaCaT cells. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.