Greenhouse gas fluxes from riparian forest soil depend on the responses of microbes to nitrogen and phosphorus additions
Greenhouse gas fluxes from riparian forest soil depend on the responses of microbes to nitrogen and phosphorus additions
复制标题
河岸森林土壤的温室气体通量取决于微生物对氮和磷添加的反应
DOI：
10.1016/j.apsoil.2021.104365
复制
发表时间：
2022-01-20
影响因子：
4.8
通讯作者：
Zhang, Kerong
中科院分区：
文献类型：
--
作者：
Chen, Youchao;Yin, Shuwei;Zhang, Kerong
人类活动极大地增加了对生物圈的氮(N)和磷(P)的输入，这可能会改变土壤的生物地球化学循环。然而，土壤温室气体(GHGs)对氮磷相互作用的响应尚不完全清楚，尤其是对于河岸森林。在这项研究中，我们测量了土壤二氧化碳(CO2)、甲烷(CH4)和一氧化二氮(N2O)的通量及其潜在驱动因素，包括土壤性质和土壤微生物。这些测量是在河岸森林的整个季节进行的，那里进行了正在进行的N和P添加实验。试验包括低N(LN，30 kg N ha(-1)年)、高N(HN，150 kg N ha(-1)年(-1))、低P(LP，30 kg P_2O_5 ha(-1)年(-1))、高P(HP，150 kg P_2O_5 ha(-1)年(-1))、低N低P(LNP)、高N高P(HNP)和对照(CK)处理。结果表明，河滨森林土壤是CO2和N2O的重要来源，也是CH4的汇。养分添加显著降低了整个季节的CO2排放(从HNP处理的-52.9%到LP处理的-44.2%)。在整个生长季节，氮肥和磷肥处理以及氮磷配合处理均显著降低了水稻对CH4的吸收。在生长季，HN处理显著增加了N2O的排放，而添加P的处理(LP、HP和LNP)则减少了N2O的排放。CO2、CH4和N2O通量分别与真菌、革兰氏阴性(GN)细菌和净硝化(NETN)速率的季节变化显著相关。总体而言，施氮和施磷分别显著降低了生长季的真菌数量和GN细菌浓度；HN施用量显著增加，而LP和HP显著降低了NETN率。我们的结果还表明，N和P的交互作用对所有温室气体都表现出拮抗作用。我们的发现表明，该河岸林土壤温室气体通量取决于微生物对N和P添加的响应，并强调了在预测土壤温室气体对全球变化的响应时，研究N和P的相互作用的必要性。
Anthropogenic activities have drastically increased nitrogen (N) and phosphorus (P) input to the biosphere, which could potentially alter soil biogeochemical cycles. However, the responses of soil greenhouse gases (GHGs) to the interactions of N and P enrichment are not fully understood, especially for riparian forests. In this study, we measured soil carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) fluxes and their potential drivers, including soil properties and soil microbes. The measurements were made across a whole season in a riparian forest, where an on-going N and P addition experiment was conducted. The experiment included low N (LN, 30 kg N ha(-1) year-( 1)), high N (HN, 150 kg N ha(-1) year(- 1)), low P (LP, 30 kg P2O5 ha(-1) year(- 1)), high P (HP, 150 kg P2O5 ha(-1) year(-1)), low N and low P (LNP), high N and high P (HNP), and a control (CK) treatment. The results show that the riparian forest soil was a significant source of CO2 and N2O and a sink for CH4. The nutrient additions significantly reduced CO2 emissions (ranging from-52.9% under HNP to-44.2% under LP treatment) across the whole season. A significant reduction in CH4 uptake was found under both N and P addition treatments and also under the combination of N and P addition during the growing season. A significant increase in N2O emissions was found under HN treatment, but decrease was found under P addition treatments (LP, HP and LNP) during the growing season. The CO2, CH4, and N2O fluxes were significantly correlated with the seasonal patterns of fungi, Gram-negative (GN) bacteria, and net nitrification (NetN) rates, respectively. Generally, N and P addition significantly reduced the amount of fungi and GN bacteria concentration, respectively; HN addition significantly increased while LP and HP decreased the NetN rates during the growing season. Our results also indicated the interactions of N and P showed antagonistic effects on all GHGs. Our findings indicate that soil GHG fluxes in this riparian forest depend on the responses of microbes to N and P additions and highlight the need to investigate the interactions of N and P in predicting the responses of soil GHGs to global change.